Characterization of North American Armillaria species by nuclear DNA content and RFLP analysis
Characterization of North American Armillaria species by nuclear DNA content and RFLP analysis
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通过核 DNA 含量和 RFLP 分析表征北美蜜环菌物种
DOI：
10.1080/00275514.2000.12061232
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
A. Vidaver
中科院分区：
文献类型：
--
作者：
Mee;N. Klopfenstein;G. I. Mcdonald;K. Arumuganathan;A. Vidaver
摘要采用流式细胞术和限制性片段长度多态性(RFLP)分析方法，对北美9种蜜环菌的26个菌株进行了核DNA含量和RFLP图谱分析。分析了三个推测为二倍体的骨裂殖吸虫A.Gemina、A.calvescens、A.sinapina、A.Mellea、A.Gallica、A.nabnon a和北美生物物种(NABS)X，以及两个推测为二倍体的NABS XI菌株。不同种的蜜环菌核DNA含量为0.11-0.17pg/核(55-84×106BP/C)。在供试的9种北美蜜环菌中，金针菇、金针菇和蜜环菌的核DNA含量相对较低(0.11-0.12pg/核)，而粗毛蜜环菌的核DNA含量相对较高(0.17pg/核)。核型DNA含量(0.13pg/核)略高于骨型、双型和蜜环型。其他物种(A.calvescens、A.sinapina、NABS X和NABS XI)具有中等的核DNA含量(约0.15pg/核)。聚合酶链式反应(PCR)和基因间隔区-1(IGS-1)的RFLP产生了9种蜜环菌的带型。除了以前报道的带型外，还出现了新的带型：A.Gemina、A.calvescens、A.mellea和A.Gallica。
Abstract Twenty-six isolates representing nine North American Armillaria species were investigated with flow cytometry and RFLP (restriction fragment length polymorphism) analyses to determine their nuclear DNA content and RFLP profile. Three putatively diploid isolates of A. ostoyae, A. gemina, A. calvescens, A. sinapina, A. mellea, A. gallica, A. nabsnona, and North American Biological Species (NABS) X were analyzed, and two putatively diploid isolates of NABS XI also were analyzed. Nuclear DNA contents of Armillaria species were 0.11–0.17 pg per nucleus (55–84 X 106 bp/C), depending on species. Among the nine North American Armillaria species tested, A. ostoyae, A. gemina, and A. mellea possessed relatively small nuclear DNA contents (0.11–0.12 pg per nucleus), whereas A. gallica possessed a relatively large nuclear DNA content (0.17 pg per nucleus). A. nabsnona has a slightly larger nuclear DNA content (0.13 pg per nucleus) than A. ostoyae, A. gemina, and A. mellea. Other species (A. calvescens, A. sinapina, NABS X, and NABS XI) possessed moderate nuclear DNA contents (ca 0.15 pg per nucleus). Polymerase chain reaction (PCR) and RFLP of the intergenic spacer region-1 (IGS-1) generated banding patterns for nine Armillaria species. In addition to previously reported banding patterns, new banding patterns are presented for A. gemina, A. calvescens, A. mellea, and A. gallica.