GENERATION AND MAPPING OF EXPRESSED SEQUENCE TAGS FROM VIRUS-INFECTED SWINE MACROPHAGES
GENERATION AND MAPPING OF EXPRESSED SEQUENCE TAGS FROM VIRUS-INFECTED SWINE MACROPHAGES
复制标题
病毒感染猪巨噬细胞表达序列标签的生成和作图
作者：
Changchun Wang;R. Hawken;E. Larson;Xuexian Zhang;L. Alexander;M. Rutherford;M. Rutherford
为了鉴定在病毒感染过程中对巨噬细胞功能有重要影响的基因，我们采用差异显示反转录(DDRT)-PCR技术捕获了猪繁殖与呼吸综合征病毒（PRRSV）感染的猪肺泡巨噬细胞的表达序列标签（ESTs）。序列分析表明，这些ESTs中约60%与已知猪ESTs或基因或其他物种的匹配序列具有显著的相似性（≥93%），同源性≥80%。为了确定染色体定位，在猪体细胞杂交或辐射杂交面板上进行了基于pcr的定位。通过猪体细胞面板或INRA-Minnesota猪辐射杂交（IMpRH）面板（LOD bbb6.0），共绘制了48个猪病毒反应ESTs。Northern blot分析证实，prrsv诱导了几种ESTs转录物表达的改变，包括2 ‘ -5 ’低聚腺苷酸合成酶和推定的双特异性磷酸酶。这些病毒反应ESTs是了解PRRSV发病机制和解剖宿主基因的良好候选基因，这些基因可能对疾病抗性有重要影响。
In an effort to identify genes that have a major effect on macrophage function during viral infection, we employed differential display reverse transcription (DDRT)-PCR to capture expressed sequence tags (ESTs) of swine alveolar macrophages infected by the porcine reproductive and respiratory syndrome virus (PRRSV). Sequence analyses showed that approximately 60% of these ESTs had significant similarity (≥93%) to known pig ESTs or genes or matched sequences from other species with homology ≥80%. To determine chromosomal localization, PCR-based mapping was performed across either swine somatic cell hybrid or radiation hybrid panels. A total of 48 porcine viral response ESTs were mapped via the swine somatic cell panel or the INRA-Minnesota porcine Radiation Hybrid (IMpRH) panel (LOD > 6.0). Northern blot analyses confirmed PRRSV-induced altered transcript expression for several ESTs, including a 2′–5′ oligoadenylate synthetase and a putative dual-specificity phosphatase. These virus-response ESTs represent good candidate genes for understanding PRRSV pathogenesis and for dissecting host genes which may have major effect on disease resistance.
影响因子：
10.5
作者：
Yamanaka, S;Poksay, KS;Innerarity, TL
通讯作者：
Innerarity, TL