Developmental stage-dependent differential gene expression of superoxide dismutase isoenzymes and their localization and physical interaction network in rice (Oryza sativa L.)
Developmental stage-dependent differential gene expression of superoxide dismutase isoenzymes and their localization and physical interaction network in rice (Oryza sativa L.)
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DOI：
10.1007/s13258-013-0138-9
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发表时间：
2014-02-01
期刊：
影响因子：
2.1
通讯作者：
Lee, Choon-Hwan
中科院分区：
文献类型：
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作者：
Nath, Krishna;Kumar, Susheel;Lee, Choon-Hwan
超氧化物歧化酶(SOD)同工酶是清除生物体中过量的活性氧的必需酶。到目前为止，SOD同工酶基因在叶片发育过程中的表达模式以及它们在亚细胞内的定位和物理相互作用网络还没有清楚地阐明。利用多种生物信息学工具，我们预测了水稻中超氧化物歧化酶亚细胞定位，并描述了它们之间的物理相互作用。利用电子计算机方法，我们获得了7个SOD基因和1个SOD铜伴侣基因存在的证据。它们的转录产物随着水稻叶片发育阶段的不同而差异表达。最后，我们进行了实时定量聚合酶链式反应(qRT-PCR)，从实验上验证了SOD基因的电子差异表达模式。在整个营养生长期，两种胞质cCuZn-超氧化物歧化酶的表达水平都很高。发现两种可塑性铁超氧化物歧化酶，它们的表达水平很低，并从营养后期开始增加。它们的表达模式非常相似，表明形成了异源二聚体。然而，它们的表达模式与拟南芥铁-超氧化物歧化酶不同。PCuZn-SOD在早期发育阶段有很高的表达，但qRT-PCR结果不同，有待于进一步研究。从SOD基因差异表达的结果中，我们可以了解每个SOD基因的作用，甚至可以在电子分析的基础上预测它们在特定情况下的作用。
Superoxide dismutase (SOD) isoenzymes are essential for scavenging excess reactive oxygen species in living organisms. So far, expression pattern of SOD isoenzymes genes along leaf development plus their sub-cellular localization and physical interaction network have not yet been clearly elucidated. Using multiple bioinformatics tools, we predicted the sub-cellular localizations of SOD isoforms and described their physical interactions in rice. Using in silico approaches, we obtained several evidences for existence of seven SOD genes and a SOD copper chaperone gene. Their transcripts were differentially expressed along with different developmental stage of rice leaf. Finally, we performed quantitative real time-polymerase chain reaction (qRT-PCR) to validate in silico differential expression pattern of SOD genes experimentally. Expression of two cytosolic cCuZn-SODs was high during the whole vegetative stage. Two plastidic Fe-SODs were found and their expression levels were very low and started to increase from the late vegetative stage. Their expression patterns were very similar to each other, indicating the formation of heterodimer. However, their expression patterns are different from those for Arabidopsis Fe-SODs. The expression of pCuZn-SOD was very high in the early developmental stage, but qRT-PCR results were different, which remains for further study. From the results on the differential expression of SOD genes, we can understand the role of each SOD gene and even predict their role under certain circumstances based on in silico analysis.