Isolation and Genome Sequencing of Hepatitis E Virus Genotype 1 Imported from India to Japan
Isolation and Genome Sequencing of Hepatitis E Virus Genotype 1 Imported from India to Japan
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DOI：
10.7883/yoken.jjid.2022.127
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发表时间：
2022-11-01
影响因子：
2.2
通讯作者：
Suzuki, Ryosuke
中科院分区：
文献类型：
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作者：
Kobayashi, Sakura;Mori, Ai;Suzuki, Ryosuke
戊型肝炎病毒（HEV）是戊型肝炎的病原体。在日本，主要检测到HEV基因型3（G3）和G4，而主要从亚洲大陆国家进口的G1是罕见的。在本研究中，我们在一名从印度访问日本的患者中检测到G1 HEV株。当PLC/PRF/5细胞（亚克隆4-21）接种来自该患者的粪便悬液时，在用过的培养基中观察到HEV RNA的积累，表明已成功地从该标本中分离出HEV。RT-PCR扩增获得了几乎完整的HEV基因组。系统进化分析表明，新分离的HEV株命名为9 HE 36 c，属于HEV G1的Ig亚型。
Hepatitis E virus (HEV) is the causative agent of viral hepatitis E. In Japan, HEV genotype 3 (G3) and G4 are predominantly detected, while Gl, mainly imported from countries in continental Asia, is rare. In the present study, we detected a G1 HEV strain in a patient who visited Japan from India. When PLC/PRF/5 cells (subclone 4-21) were inoculated with a stool suspension from this patient, accumulation of HEV RNA was observed in the spent culture medium, indicating that HEV had been successfully isolated from this specimen. A nearly complete HEV genome was obtained by RT-PCR amplification. Phylogenetic analyses revealed that the newly isolated HEV strain, designated 9HE36c, belongs to subtype lg of HEV Gl.