In vitro effect of synthetic pyocyanine on neutrophil superoxide production.
In vitro effect of synthetic pyocyanine on neutrophil superoxide production.
复制标题
合成绿脓素对中性粒细胞超氧化物产生的体外影响。
DOI：
10.1128/iai.55.3.559-563.1987
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Sorensen,RU
中科院分区：
文献类型：
--
作者：
Miller,KM;Dearborn,DG;Sorensen,RU
绿青素是一种由铜绿假单胞菌产生的低分子吩嗪类色素，此前已被证明能强烈抑制人类淋巴细胞的生成。我们现在报道，合成的花青素也可以剂量依赖的方式影响人外周血多形核白细胞(PMN)产生超氧化物。用佛波醇肉豆蔻酸酯(PMA)刺激中性粒细胞产生超氧化物，在存在和不存在花青碱、吩嗪和三氟拉嗪的情况下进行了测量。当浓度为50微米时，绿青素对超氧化物生成的抑制作用为PMA控制值的28.9+/-2.8%，而当较低浓度为1微米时，超氧化物的生成显著增加(为PMA控制值的203+/-31.7%)。吩嗪是绿菁的三环母体化合物，只有很小的影响。三氟拉嗪在1微米以上对超氧阴离子产生有明显的抑制作用。在没有PMA的情况下，这些化合物都不能诱导超氧化物的产生。与吩噻嗪不同的是，所有浓度的花青素对中性粒细胞酶的释放几乎没有影响。铜绿假单胞菌吩嗪色素对多形核吞噬细胞的影响是显著的，因为在感染部位抑制宿主PMN功能可能导致无效的细菌杀灭，而增强PMN功能可能导致更大的组织损伤。这两种可能性并不是相互排斥的，可能会共存，这取决于当地的花青素浓度。
Pyocyanine, a low-molecular-weight phenazine pigment produced by Pseudomonas aeruginosa, has previously been shown to strongly inhibit human lymphocyte blastogenesis. We now report that synthetic pyocyanine can also affect the generation of superoxide by human peripheral blood polymorphonuclear leukocytes (PMNs) in a dose-dependent manner. Superoxide production by PMNs stimulated with phorbol myristate acetate (PMA) was measured in the presence and absence of pyocyanine, phenazine, and trifluoperazine, a phenothiazine of similar chemical structure to the phenazine pigments. Pyocyanine at 50 microM inhibited superoxide production to 28.9 +/- 2.8% of PMA control values, whereas at the lower concentration of 1 microM, the production of superoxide was significantly enhanced (203 +/- 31.7% of PMA control values). Phenazine, the tricyclic parent compound of pyocyanine, had only a minor effect. Trifluoperazine had a marked inhibitory effect on superoxide generation at concentrations above 1 microM. None of the compounds induced superoxide generation in the absence of PMA. Pyocyanine at all concentrations, unlike phenothiazines, had very little effect on the release of neutrophil granule enzymes. The effect of P. aeruginosa phenazine pigments on polymorphonuclear phagocytes is of significance, since inhibition of host PMN function at sites of infection could result in ineffective bacterial killing, whereas enhanced PMN function could lead to greater tissue damage. These two possibilities are not mutually exclusive and may coexist depending on local pyocyanine concentrations.