Elevation of dopamine level reduces host-seeking activity in the adult female mosquito Aedes albopictus.
Elevation of dopamine level reduces host-seeking activity in the adult female mosquito Aedes albopictus.
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DOI：
10.1186/1756-3305-5-92
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发表时间：
2012-05-10
影响因子：
3.2
通讯作者：
Miake F
中科院分区：
文献类型：
--
作者：
Fukumitsu Y;Irie K;Satho T;Aonuma H;Dieng H;Ahmad AH;Nakashima Y;Mishima K;Kashige N;Miake F
蚊媒病毒通过雌蚊的吸血行为传播给人类宿主。雌蚊寻找宿主吸血（寻找宿主行为）。为了防止病毒感染，了解病毒如何调节寄主寻找行为是很重要的。多巴胺（DA）在昆虫的中枢神经系统中起着调节多种行为的神经介质的作用。在雌蚊中，当羽化后头部DA水平下降时，寻找宿主的行为会增加。然而，尚不清楚DA是否直接调节雌蚊的寻宿主行为。本研究的目的是研究头部DA水平的变化是否会影响成年雌蚊白纹伊蚊（伊蚊）的寻找宿主活动。蚊)。我们用高效液相色谱法确定了l-β-3,4-二羟基苯丙氨酸（l- dopa）组（l- dopa组）与未处理的对照组（对照组）的头部DA升高后，比较了一组初生雌性成虫的寻宿主行为。左旋多巴组的头部DA含量在所有时间内均显著高于对照组。在6天的实验期间，对照组的找宿主活性逐渐增加。相比之下，左旋多巴组寻找宿主的活性没有增加。因此，在羽化后第3 ~ 6天，左旋多巴组的寻宿主活性显著低于对照组。结果表明，DA水平升高可降低成年雌蚊寻找宿主的活性。蚊。
Mosquito-borne viruses are transmitted to human hosts via blood-feeding behavior of female mosquitoes. Female mosquitoes seek a host to take blood meals (host-seeking behavior). In order to prevent virus infections, it is important to understand how they modulate host-seeking behavior. Dopamine (DA) in the central nervous system acts as a neuromediator that regulates a variety of behaviors in insects. In female mosquitoes, host-seeking behavior increases when DA levels in the head decline after emergence. However, it remains unclear whether DA directly modulates host-seeking behavior in female mosquitoes. The aim of this study was to examine whether changes in DA levels in the head affects host-seeking activity in the adult female mosquito Aedes albopictus (Ae. albopictus). We compared host-seeking behavior in one group of emerging female adults treated with l-β-3,4-dihydroxyphenylalanine (l-DOPA), the precursor of DA, (l-DOPA group), with that in an untreated control (control group) after confirming elevation of head DA in l-DOPA group by using high-performance liquid chromatography. The content of head DA in l-DOPA group significantly remained higher than that in controls on all days examined. The host-seeking activity in the control group showed a gradual increase over the 6-day experimental period. In contrast, there was no such increase in the host-seeking activity in the l-DOPA group. Therefore, the host-seeking activity of l-DOPA group was significantly lower than that of the controls between day 3 and 6 post-emergence. Our results indicate that elevation of DA level reduces host-seeking activity in adult female mosquito Ae. albopictus.