HU-LY-M5 - A UNIQUE ANTIGEN PHYSICALLY ASSOCIATED WITH HLA MOLECULES
HU-LY-M5 - A UNIQUE ANTIGEN PHYSICALLY ASSOCIATED WITH HLA MOLECULES
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DOI：
10.1016/0198-8859(83)90002-2
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发表时间：
1983-01-01
期刊：
影响因子：
2.7
通讯作者：
MCKENZIE, IFC
中科院分区：
文献类型：
--
作者：
SPARROW, RL;MCKENZIE, IFC
本文报道了用小鼠单克隆抗体E4.3检测到的一种新的人细胞表面抗原（Hu Ly-m5）。Hu Ly-m5抗原的组织分布与HLA抗原相似，但并不存在于所有骨髓细胞和[人]U266骨髓瘤细胞系中，而在HLA阴性的[人白血病]K562细胞系中表达。Hu - Ly-m5抗原对HLA分子具有一定的亲和力，作为2个实体的cocap， Hu - Ly-m5抗原与HLA抗原在anti- β上结合。2-微球蛋白免疫吸收柱；这些抗原配合物经受不住共沉淀法的考验。尽管有相似之处，Hu Ly-m5和HLA抗原是不同的分子实体，Hu Ly-m5由2个表观分子量为69和60 K[千道尔顿]的条带组成，而HLA由HLA H链的43和12 K带和。β组成。分别2-microglobulin。Hu Ly-m5抗原的功能尚不清楚，但未证实其参与细胞毒性t淋巴细胞对流感病毒感染细胞的反应。所描述的两个特性（明显的分子量和与HLA抗原的物理关联）表明，Hu Ly-m5抗原可能是一种病毒编码的蛋白。
A new human cell surface antigen (Hu Ly-m5) detected by a murine monoclonal antibody (E4.3) is described. The tissue distribution of the Hu Ly-m5 antigen is similar to the HLA antigens, but it is not present on all bone marrow cells nor the [human] U266 myeloma cell line, and is expressed on the HLA-negative K562 [human leukemia] cell line. The Hu Ly-m5 antigen has some affinity for HLA molecules as the 2 entities cocap and the Hu Ly-m5 antigen copurifies with the HLA antigens on an anti-.beta.2-microglobulin immunoabsorbent column; the antien complexes did not withstand the procedures used for coprecipitation. Despite their similarities, the Hu Ly-m5 and HLA antigens are distinct molecular entities, Hu Ly-m5 consists of 2 bands of apparent MW 69 and 60 K [kilodaltons] while HLA is comprised of the 43 and 12 K bands of the HLA H chain and .beta.2-microglobulin, respectively. The function of the Hu Ly-m5 antigen is unknown, but no involvement in the cytotoxic T-lymphocyte response to influenza virus-infected cells could be demonstrated. The 2 properties described (apparent MW and physical association with the HLA antigens) suggests that the Hu Ly-m5 antigen may be a viral-encoded protein.