Three-dimensional characteristics of the endoplasmic reticulum in the columnar cells of the rat small intestine: an electron microscopy study in thick section.
Three-dimensional characteristics of the endoplasmic reticulum in the columnar cells of the rat small intestine: an electron microscopy study in thick section.
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大鼠小肠柱状细胞内质网的三维特征：厚切片电子显微镜研究。
DOI：
10.1002/aja.1001670406
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发表时间：
1983
影响因子：
--
通讯作者：
M. Bergeron
中科院分区：
文献类型：
--
作者：
G. Thiéry;P. Gaffiero;M. Bergeron
本文用特异性封闭染色技术和标准透射电镜技术对大鼠空肠柱状细胞的内质网进行了研究。一个新的三维模型，立体对照片的分析的基础上，提出了这个模型表明，内质网可能构成一个跨细胞的路线。在用四氧化锇浸渍5天后制备柱状细胞的厚切片（0.5-1微米），并在80-100 kV下通过标准透射电子显微镜检查。在细胞从隐窝到绒毛顶部的细胞上升过程中，ER的进化趋向于更大的复杂性。在Lieberkühn隐窝的底部，没有明确的组织被注意到，最常见的是只有核膜和小管成分被染色。成熟柱状细胞的内质网形成一个连续的小管和有孔囊的网络;它从微绒毛下方的顶端延伸到侧质膜和基底质膜，并向核膜发送许多突起。在细胞的基底部，在核下面，ER主要由管状小管组成，而大多数囊泡在核上部观察到。小管的直径为40-60 nm。有孔囊似乎形成围绕线粒体的连续管状结构;囊具有25-40 nm的厚度并且具有35-60 nm的不规则穿孔。最后，在某些细胞中，内质网似乎显示功能差异，反映了在绒毛的同一部分中，邻近染色良好的细胞的细胞中没有反应;因此锇浸渍技术似乎是研究ER组织的有效工具。
The endoplasmic reticulum (ER) of the columnar cells of the rat jejunum was studied with a specific block-staining technique and standard transmission electron microscopy. A new three-dimensional model, based on the analysis of stereo pair photographs, is proposed; this model suggests that the endoplasmic reticulum may constitute a transcellular route. Thick sections (0.5-1 micron) of columnar cells were made after a 5-day impregnation with osmium tetroxide and were examined by standard transmission electron microscopy at 80-100 kV. The evolution of the ER during the cellular ascent of cells from the crypt to the top of the villus is toward a greater complexity. At the base of the crypt of Lieberkühn, no definite organization is noted, and most often only the nuclear envelope and canalicular elements are stained. The endoplasmic reticulum of the mature columnar cells forms a continuous network of canaliculi and fenestrated saccules; it extends from the apex, below the microvilli, to the lateral and basal plasma membranes, and sends many projections to the nuclear envelope. In the basal part of the cell, below the nucleus, the ER consists mainly of tubular canaliculi, whereas mostly saccules are observed in the supranuclear part. The canaliculi have a diameter of 40-60 nm. Fenestrated saccules appear to form a continuous tubular structure surrounding mitochondria; the saccules have a thickness of 25-40 nm and possess irregular perforations of 35-60 nm. Finally, in some cells, the endoplasmic reticulum seems to show functional differences as reflected by the absence of reaction in cells adjacent to well-stained cells in the same part of the villus; thus the osmium impregnation technique appears to be a valid tool for studying the ER organization.