Distribution pattern of versican, link protein and hyaluronic acid in the rat periodontal ligament during experimental tooth movement
Distribution pattern of versican, link protein and hyaluronic acid in the rat periodontal ligament during experimental tooth movement
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DOI：
10.1034/j.1600-0765.2002.90770.x
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发表时间：
2002-02-01
影响因子：
3.5
通讯作者：
Yamauchi, M
中科院分区：
文献类型：
--
作者：
Sato, R;Yamamoto, H;Yamauchi, M
牙周膜的快速重塑能力是正畸牙齿移动的基础。在牙齿移动过程中，基质蛋白多糖(PGs)可能在维持和修复牙周膜的空间、力学和生物学方面发挥重要作用。本研究的目的是用组织化学和免疫组织化学方法研究大鼠磨牙移动过程中大分子透明质酸(HA)结合蛋白多糖、Versican、连接蛋白(LP)和HA在磨牙PDL中的分布。实验牙齿移动按Waldo方法进行。组织学观察，治疗1d后，受压侧可见成纤维细胞、胶原纤维减少、渗出性改变或水肿等退行性改变，应变侧可见成纤维细胞、胶原纤维增殖等进行性改变。在牙齿移动后3天。两侧均未观察到退行性或渐进性改变。使用针对VERSICAN核心蛋白或LP的单抗，这两个分子的阳性免疫反应在整个PDL中一直被观察到。然而，实验力作用于牙齿后，Verscan和LP的免疫染色仅在压缩侧显著增强，而在应变侧明显减弱。受压侧的强度在施力后1d最强，此后逐渐减弱。在实验牙齿移动过程中，两侧HA无明显变化。因为在PDL中存在HA。在受压侧表达的大量Verscan和LP可能通过与HA结合而产生大的水化聚集体，从而分散施加在该组织上的压缩力。
The ability of the periodontal ligament (PDL) to rapidly remodel is the basis of orthodontic tooth movement. During the tooth movement, matrix proteoglycans (PGs) may play important roles in spatial, mechanical and biological aspects for the maintenance and repair of the PDL. The aim of this study was to characterize the distribution of a large hyaluronic acid (HA)-binding proteoglycan, versican, link protein (LP) and HA in the rat molar PDL during experimental tooth movement by histochemical and immunohistochemical methods. Experimental tooth movement was performed according to Waldo's method. Histologically, regressive changes, such as decrease of fibroblasts and collagen fibers and exudative change or edema were observed in the compressive side and progressive changes, such as proliferation of fibroblasts and collagen fibers, in the strain side one day after treatment. By 3 days after tooth movement. regressive or progressive changes were not observed in either side. Using monoclonal antibodies specific to versican core protein or LP, the positive immunoreactivity for both molecules was constantly observed throughout the PDL. After the experimental force was applied to the tooth, however, the immunostainings of versican and LP became significantly intense only in the compressive side but decreased in the strain side. The intensity in the compressive side was strongest one day after the force was applied and gradually diminished thereafter. HA of both sides did not change during experimental tooth movement. Since HA is present in the PDL. large amounts of versican and LP expressed in the compressive side may create large hydrated aggregates via their association with HA that dissipates the compressive force applied to this tissue.