Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure.
Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure.
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DOI：
10.1016/j.scitotenv.2007.06.026
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
V. de Vlaming;A. Biales;D. Riordan;D. Markiewicz;R. Holmes;P. Otis;R. Zander;J. Lazorchak
中科院分区：
文献类型：
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作者：
V. de Vlaming;A. Biales;D. Riordan;D. Markiewicz;R. Holmes;P. Otis;R. Zander;J. Lazorchak
在过去的15年里，对水生物种内分泌系统功能受到破坏的化学物质的关注日益增加。然而，对加州淡水生态系统中雌激素内分泌干扰物（EEDCs）的监测却很少受到重视。目的是利用实时逆转录酶聚合酶链式反应（Q-RT PCR）技术定量测定虹鳟鱼幼鱼肝脏中卵黄蛋白原（Vtg） mRNA，以筛选地表水样品的雌激素活性。从加利福尼亚中央谷地和北部地区的地表水中采集的113个环境水样暴露在鱼肝脏中的Vtg mRNA分析表明，6个样本（占总数的5%）可能含有EEDCs。6个样品诱导了Vtg mRNA的边际升高，但有统计学意义。没有环境水样引起与阳性对照相当的Vtg mRNA反应（所有反应都不到阳性对照反应的2%）。因此，这些样品中的EEDC浓度很低（达到或接近该程序的阈值），或者结果可能包括假阳性。为了对EEDC的发生进行更明确的评估，建议在观察到有统计学意义但雌激素活性较弱的部位进行随访筛查。总体而言，结果显示，大多数加州地表水测试低于EEDC检测阈值浓度的筛选程序所使用的。
Concern regarding the occurrence of chemicals that disrupt endocrine system functions in aquatic species has heightened over the last 15 years. However, little attention has been given to monitoring for estrogenic endocrine disrupting chemicals (EEDCs) in California's freshwater ecosystems. The objective was to screen surface water samples for estrogenic activity using vitellogenin (Vtg) mRNA quantification in livers of juvenile rainbow trout by real-time reverse transcriptase polymerase chain reaction (Q-RT PCR). Vtg mRNA analysis of livers from fish exposed to 113 ambient water samples collected from surface waters in California's Central Valley and northern area indicated that six samples (5% of total) may have contained EEDCs. The six samples induced marginal, but statistically significant, increases of Vtg mRNA. No ambient water sample evoked Vtg mRNA responses equivalent to those in positive controls (all responses were less than 2% of the positive control response). Thus, EEDC concentrations in these samples were low (at or near the threshold for the procedure) or results may have included false positives. To establish a more definitive assessment of EEDC occurrence, follow-up screening at sites where statistically significant, but weak, estrogenic activity was observed is recommended. Overall, results reveal that a majority of the California surface waters tested were below EEDC detection threshold concentration for the screening procedure utilized.