The nucleus serves as the pacemaker for the cell cycle.
The nucleus serves as the pacemaker for the cell cycle.
复制标题
DOI：
10.7554/elife.59989
复制
发表时间：
2020-12-07
期刊：
影响因子：
7.7
通讯作者：
Ferrell JE Jr
中科院分区：
文献类型：
--
作者：
Afanzar O;Buss GK;Stearns T;Ferrell JE Jr
有丝分裂是一个戏剧性的过程，影响细胞的所有部分。它由一个振荡器驱动，振荡器的各种成分位于细胞核、中心体和细胞质中。原则上，具有最快内在节律的细胞位置应该充当该过程的起搏器。在这里，我们追踪了非洲爪蟾卵提取物中有丝分裂进入和退出时发生的微管蛋白聚合和解聚波，追溯到它们的起源。我们发现，有丝分裂通常是在精子来源的核及其伴随的中心体开始。在这些起始点的细胞周期比浸提液中最慢的区域快约20%。从噬菌体DNA产生的细胞核，它不具有中心体，也作为触发波源，但在没有细胞核的情况下，纯化的中心体没有。我们的结论是，细胞核加速有丝分裂进入，并建议它作为一个起搏器的细胞周期。
Mitosis is a dramatic process that affects all parts of the cell. It is driven by an oscillator whose various components are localized in the nucleus, centrosome, and cytoplasm. In principle, the cellular location with the fastest intrinsic rhythm should act as a pacemaker for the process. Here we traced the waves of tubulin polymerization and depolymerization that occur at mitotic entry and exit in Xenopus egg extracts back to their origins. We found that mitosis was commonly initiated at sperm-derived nuclei and their accompanying centrosomes. The cell cycle was ~20% faster at these initiation points than in the slowest regions of the extract. Nuclei produced from phage DNA, which did not possess centrosomes, also acted as trigger wave sources, but purified centrosomes in the absence of nuclei did not. We conclude that the nucleus accelerates mitotic entry and propose that it acts as a pacemaker for cell cycle.