Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium
Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium
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DOI：
10.1038/s41598-018-27540-9
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发表时间：
2018-06-19
影响因子：
4.6
通讯作者：
Vermeir, Pieter
中科院分区：
文献类型：
--
作者：
De Leersnyder, Ilse;De Gelder, Leen;Vermeir, Pieter
许多研究已经调查了银离子和银纳米材料对多种环境和临床相关细菌的抗菌作用。然而，报告了相互矛盾的结果，其中抑制浓度变化10倍。本研究调查了结果的这种差异是否可归因于实验条件的差异，特别是微生物生长培养基。B。将枯草杆菌暴露于含有不同浓度的一些常用培养基组分的液体生长培养基中的500 μ g L-1 Ag+：胰蛋白胨、酵母提取物、Cl-和S2-。通过三种互补分析技术研究了毒性作用：（i）分析通过光密度测量获得的生长曲线，（ii）使用流式细胞术，和（iii）通过透射电子显微镜。银离子对B的毒性。随着胰蛋白胨、酵母提取物或S2-的存在，枯草杆菌的数量减少。本研究表明，培养基组成（很少被认为是细菌毒性研究中的重要实验因素）对观察到的银对B的毒性具有深远影响。枯草芽孢杆菌
Numerous studies have investigated the antibacterial effect of both silver ions and silver nanomaterials on a large diversity of environmentally and clinically relevant bacteria. However, contradictory results are reported in which inhibition concentrations were varying by a 10-fold. This study investigated whether this variance in results could be attributed to the difference in experimental conditions, especially the microbial growth medium. B. subtilis was exposed to 500 mu g L-1 Ag+ in liquid growth media with different concentrations of some commonly used media components: tryptone, yeast extract, Cl-, and S2-. The toxic effect was investigated by means of three complementary analysis techniques: (i) analyzing the growth curves obtained by optical density measurements, (ii) using flow cytometry, and (iii) by transmission electron microscopy. The silver ion toxicity towards B. subtilis decreased as more tryptone, yeast extract, or S2- was present. This study demonstrates that the medium composition, rarely acknowledged as an important experimental factor in bacterial toxicity studies, has a profound impact on the observed silver toxicity towards B. subtilis.