Different patterns of genealogical relationships found in the two major QTLs causing reduction of seed shattering during rice domestication
Different patterns of genealogical relationships found in the two major QTLs causing reduction of seed shattering during rice domestication
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DOI：
10.1139/g07-051
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发表时间：
2007-08-01
期刊：
影响因子：
3.1
通讯作者：
Sano, Yoshio
中科院分区：
文献类型：
--
作者：
Onishi, Kazumitsu;Takagi, Kyoko;Sano, Yoshio
研究了引起落粒率降低的3个数量性状基因座（qSH 1、qSH 3和qSH 4）在水稻驯化过程中的相对重要性。与qSH 3位点相比，qSH 1和qSH 4位点对降低落粒率有明显的作用。精细定位和序列分析表明，qSH 1和qSH 4位点与最近报道的基因相同。即使qSH 3和qSH 4基因座存在落粒等位基因，qSH 1的非落粒等位基因也会将落粒表型急剧改变为非落粒表型，表明qSH 1对其他基因座具有遗传上位性。在qSH 1和qSH 4区域之间比较序列多样性的降低水平。水稻亚种（Oryza sativa subsp.）japonica与O.青indica，尽管在2个亚种中qSH 4区域的多样性水平同样降低。基于侧翼位点组合序列的系统发育重建显示了两个亚种不同的模式。这2个亚种在qSH 4基因上形成了一个分支，而在qSH 1基因上则分成了不同的谱系，表明这2个位点在水稻驯化过程中有不同的历史。
The three quantitative trait loci (qSH1, qSH3, and qSH4) causing reduction of seed shattering were investigated to examine their relative importance during rice domestication. The qSH1 and qSH4 loci showed a distinct effect on the reduction of shattering, compared with the qSH3 locus. Fine mapping and sequence analysis strongly suggested that the qSH1 and qSH4 loci are the same as the recently reported genes. A non-shattering allele at qSH1 drastically changed the shattering phenotype to a non-shattering phenotype even in the presence of shattering alleles at the qSH3 and qSH4 loci, showing that qSH1 is genetically epistatic to the other loci. The level of the reduction in sequence diversity was compared between the qSH1 and qSH4 regions. The sequence diversity was severely reduced in the qSH1 region of Oryza sativa subsp. japonica compared with that of O. sativa subsp. indica, despite that the level of diversity was similarly reduced at the qSH4 region in the 2 subspecies. Phylogenetic reconstruction based on the combined sequences of the flanking sites showed different patterns in the 2 subspecies. The 2 subspecies formed a single clade with respect to qSH4, whereas they were separated into different lineages with respect to qSH1, suggesting that these loci had different histories during rice domestication.