Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming
Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming
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DOI：
10.1038/s41467-020-16036-8
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发表时间：
2020-05-05
影响因子：
16.6
通讯作者：
Mackenzie, Sally A.
中科院分区：
文献类型：
--
作者：
Yang, Xiaodong;Sanchez, Robersy;Mackenzie, Sally A.
MSH 1是一种植物特异性蛋白。MSH 1的RNAi抑制导致发育和应激反应途径的表型变异。RNAi转基因的分离产生具有多代遗传的非遗传msh 1“记忆”。第一代记忆与非记忆的比较，以及六代遗传研究，确定了基因相关的，可遗传的甲基化重新模式。全基因组甲基化分析与RNAseq和基于网络的富集研究相结合，可以识别改变的生物钟网络，以及与昼夜节律控制交叉的植物激素和应激反应途径。共有373个差异甲基化位点，包括这些网络是足够的，以区分记忆非记忆全同胞。甲基化抑制剂5-氮杂胞苷减少了记忆和野生型之间在生长、基因表达和甲基化模式方面的差异。msh 1重编程依赖于功能性组蛋白去乙酰化酶6和甲基转移酶MET 1，而向记忆的过渡需要RNA指导的DNA甲基化途径。这种表型可塑性的系统可以作为一个有效的模型来定义加速植物适应环境变化。MSH 1 RNAi转基因的分离产生非遗传记忆，在拟南芥中显示跨代遗传。在这里，作者比较了记忆和非记忆全同胞后代，以显示DNA甲基化重编程的参与，涉及RdDM途径，在过渡到可遗传的记忆状态。
MSH1 is a plant-specific protein. RNAi suppression of MSH1 results in phenotype variability for developmental and stress response pathways. Segregation of the RNAi transgene produces non-genetic msh1 'memory' with multi-generational inheritance. First-generation memory versus non-memory comparison, and six-generation inheritance studies, identifies gene-associated, heritable methylation repatterning. Genome-wide methylome analysis integrated with RNAseq and network-based enrichment studies identifies altered circadian clock networks, and phytohormone and stress response pathways that intersect with circadian control. A total of 373 differentially methylated loci comprising these networks are sufficient to discriminate memory from nonmemory full sibs. Methylation inhibitor 5-azacytidine diminishes the differences between memory and wild type for growth, gene expression and methylation patterning. The msh1 reprogramming is dependent on functional HISTONE DEACETYLASE 6 and methyltransferase MET1, and transition to memory requires the RNA-directed DNA methylation pathway. This system of phenotypic plasticity may serve as a potent model for defining accelerated plant adaptation during environmental change. Segregation of an MSH1 RNAi transgene produces non-genetic memory that displays transgenerational inheritance in Arabidopsis. Here, the authors compare memory and non-memory full-sib progenies to show the involvement of DNA methylation reprogramming, involving the RdDM pathway, in transition to a heritable memory state.