Immunoregulatory roles of adhesive interactions between lymphocytes and gingival fibroblasts
Immunoregulatory roles of adhesive interactions between lymphocytes and gingival fibroblasts
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DOI：
10.1111/j.1600-0765.1997.tb01390.x
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Okada, H
中科院分区：
文献类型：
--
作者：
Murakami, S;Shimabukuro, Y;Okada, H
慢性成人牙周炎通常以炎症细胞在牙周血管外结缔组织中的积聚为特征。为了揭示淋巴细胞在牙周病变中的迁移和滞留是如何调节的，我们重点研究了淋巴细胞与人牙龈成纤维细胞（HGF）之间粘附相互作用的分子基础。在这项研究中？我们研究了细胞粘附分子参与淋巴细胞和HGF之间的粘附相互作用。我们发现，活化的淋巴细胞强烈结合HGF和VLA整合素，细胞外基质受体，发挥关键作用的结合。有趣的是，我们首次发现淋巴细胞上的CD 44分子（透明质酸受体）也参与淋巴细胞-HGF相互作用，并且锚定在HGF表面的透明质酸作为CD 44的配体发挥作用。此外，当用IL-1等炎症细胞因子刺激HGF时。TNF-α和IFN-γ的作用下，淋巴细胞与HGF的结合亲和力显著增加，粘附主要由LFA-1/ICAM-1途径介导。然后，我们研究了淋巴细胞-HGF相互作用是否可能导致HGF活化的可能性。当HGF与淋巴细胞直接作用3 h时，HGF中IL-1 β mRNA的表达明显增加。提示淋巴细胞与HGF之间的粘附作用至少是通过VLA整合素、LFA-1/ICAM-1和CD 44/hyaluronate介导的，异型的细胞间相互作用可以相互引起细胞内信号转导。
Chronic adult periodontitis is usually characterized by iuflammatory cell accumulation in the extravascular periodontal connective tissue. In order to reveal how the lymphocyte migration and retention in periodontal lesions is regulated, we have focused on the molecular basis for the adhesive interactions between lymphocytes and human gingival fibroblasts (HGF). In this study? we investigated the involvement of cell adhesion molecules in adhesive interactions between lymphocytes and HGF. We found that activated lymphocytes bound strongly to HGF and VLA integrins, extracellular matrix receptors, play crucial roles in the binding. Interestingly, we first revealed that CD44 molecules (hyaluronate receptor) on lymphocytes also participated in lymphocyte-HGF interactions and that hyaluronate anchored on the surface of HGF functioned as the ligand for CD44. In addition, when HGF were stimulated with inflammatory cytokines such as IL-1. TNF-alpha and IFN gamma, the binding avidity between lymphocytes and HGF was significantly increased and the adhesion was mainly mediated by LFA-1/ICAM-1 pathway. We then examined the possibility whether lymphocytct-HGF interaction may cause activation of HGF. When HGF directly interacted with lymphocytes for 3 h, IL-1 beta mRNA expression was clearly increased in HGF. These findings suggested that the adhesive interactions between lymphocytes and HGF was mediated at least by VLA integrins, LFA-1/ICAM-1 and CD44/hyaluronate and that the heterotypic cell-cell interactions could mutually cause intracellular signal transduction.