Use of endothelial cells containing superparamagnetic microspheres to improve endothelial cell delivery to arterial surfaces after angioplasty
Use of endothelial cells containing superparamagnetic microspheres to improve endothelial cell delivery to arterial surfaces after angioplasty
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DOI：
10.1016/s1051-0443(99)70458-6
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发表时间：
1999-02-01
影响因子：
2.9
通讯作者：
Vitali, NJ
中科院分区：
文献类型：
--
作者：
Consigny, PM;Silverberg, DA;Vitali, NJ
目得：本研究的目的是确定球囊扩张动脉的管腔表面是否可以使用正常内皮细胞（EC）和含超顺磁性微球的内皮细胞（MagEC）分别覆盖重力依赖性和独立的动脉表面来周向地再内皮化。材料和方法：兔微血管内皮细胞吞噬白蛋白包被的超顺磁性聚苯乙烯微球后获得MagEC，微球内化对细胞粘附的影响在体外通过比较EC和MagEC在粘附到纤连蛋白和暴露于剪切应力后的细胞保留方面的时间过程来确定。通过用双球囊导管将荧光标记的EC和MagEC递送到球囊扩张的动脉，在动脉上放置磁体，并轴向旋转兔来进行体内再内皮化。动脉横截面的腔内覆盖率估计epifluorescence microscopic.RESULTS：在重力的影响下，在体外细胞粘附到纤连蛋白后5，10和15分钟是相似的EC（34%，74%和70%）和MagEC（40%，56%和93%）。暴露于剪切应力后的体外细胞保留（25达因/厘米（2））更大（P <0.05），对于EC比对于MagEC（82%对69%），体内使用EC加MagEC导致细胞递送几乎是周向的。EC和MagEC的混合物的递送与动物旋转和磁场的组合提供了EC到管腔的几乎周向递送。细胞中超顺磁性微球的存在不妨碍细胞粘附，但在暴露于流体剪切力后确实减少细胞滞留。
PURPOSE: The purpose of this study was to determine if the luminal surface of balloon-dilated arteries can be re-endothelialized circumferentially with use of normal endothelial cells (ECs) and superparamagnetic microsphere-containing endothelial cells (MagECs) to cover gravity-dependent and independent arterial surfaces, respectively.MATERIALS AND METHODS: MagECs were obtained after phagocytosis of albumin-coated superparamagnetic polystyrene microspheres by rabbit microvascular ECs, The effect of microsphere internalization on cell adhesion was determined in vitro by comparing ECs and MagECs in terms of time courses of adhesion to fibronectin and cell retention after exposure to a shear stress. In vivo re-endothelialization was performed by delivering fluorescently labeled ECs and MagECs to a balloon-dilated artery with a double-balloon catheter, placing a magnet over the artery, and rotating the rabbit axially. Endoluminal coverage of arterial cross-sections was estimated by epifluoresence microscopy.RESULTS: Under the influence of gravity, in vitro cell adhesion to fibronectin after 5, 10, and 15 minutes was similar for the ECs (34%, 74%, and 70%) and MagECs (40%, 56%, and 93%). In vitro cell retention after exposure to a shear stress (25 dynes/cm(2)) was greater (P < .05) for ECs than for MagECs (82% vs 69%), Use of ECs plus MagECs in vivo resulted in cell delivery that was nearly circumferential.CONCLUSIONS: Delivery of a mixture of ECs and MagECs in combination with animal rotation and a magnetic field provide nearly circumferential delivery of ECs to the luminal surface of balloon-dilated arteries, The presence of superparamagnetic microspheres in cells does not impede cell adhesion but does decrease cell retention after exposure to a fluid shear.