Immediate Impact of Hurricane Lane on Microbiological Quality of Coastal Water in Hilo Bay, Hawaii
Immediate Impact of Hurricane Lane on Microbiological Quality of Coastal Water in Hilo Bay, Hawaii
复制标题
飓风莱恩对夏威夷希洛湾沿海水域微生物质量的直接影响
DOI：
10.1021/acs.est.0c07082
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发表时间：
2021
影响因子：
11.4
通讯作者：
Yan, Tao
中科院分区：
文献类型：
--
作者：
Saingam, Prakit;Li, Bo;Sung, Shihwu;Yan, Tao
飓风和相关的雨水径流事件预计将极大地影响沿海海水水质，但很少有人知道他们对近岸水的微生物质量的直接影响。这项研究在飓风莱恩影响后立即对希洛湾进行了采样，以了解粪便指示肠球菌、常见粪便病原体和抗生素耐药基因（ARG）丰度和多样性的空间和时间变化。在7天内从7个采样点采集水样并进行分析，结果表明，总体微生物水质参数[肠球菌几何平均值（GM）：6-22 cfu/100 mL]符合水质标准，时间动态表明水质持续恢复。然而，观察到相当大的空间变化，与污染最严重的网站表现出受损的水质（GM = 144 cfu/100毫升）。在污染最严重的地点，肠球菌种群也表现出不同的基因型组成。虽然未检测到典型粪便病原体的标记基因（沙门氏菌的invA、弯曲杆菌的hipO、嗜肺军团菌的mip和肠致病性大肠杆菌的deaeA），但检测到高水平的各种ARG（ermB、qurS、tetM、blaTEM和sul 1）和整合子相关整合酶intI 1。了解微生物水质在细粒度上的时空变化对于平衡沿海水的经济和娱乐用途以及在重大飓风事件影响后保护公众健康非常重要。
Hurricanes and associated stormwater runoff events are expected to greatly impact coastal marine water quality, yet little is known about their immediate effects on microbiological quality of near-shore water. This study sampled Hilo Bay immediately after the impact of Hurricane Lane to understand the spatial and temporal variations of the abundance and diversity of fecal indicator enterococci, common fecal pathogens, and antibiotic resistance genes (ARGs). Water samples from seven sampling sites over 7 days were collected and analyzed, which showed that the overall microbiological water quality parameters [enterococci geometric mean (GM): 6–22 cfu/100 mL] fell within water quality standards and that the temporal dynamics indicated continuing water quality recovery. However, considerable spatial variation was observed, with the most contaminated site exhibiting impaired water quality (GM = 144 cfu/100 mL). TheEnterococcuspopulation also showed distinct genotypic composition at the most contaminated site. Although marker genes for typical fecal pathogens (invAforSalmonella,hipOforCampylobacter,mipforLegionella pneumophila, andeaeAfor enteropathogenicEscherichia coli) were not detected, various ARGs (ermB,qurS,tetM,blaTEM, andsul1) and integron-associated integraseintI1 were detected at high levels. Understanding the temporal and spatial variation of microbiological water quality at fine granularity is important for balancing economic and recreational uses of coastal water and the protection of public health post the impact of major hurricane events.