Fluorescence-based nitric oxide detection
Fluorescence-based nitric oxide detection
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DOI：
10.1007/0-387-23335-0_4
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Lippard, SJ
中科院分区：
文献类型：
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作者：
Hilderbrand, SA;Lim, MH;Lippard, SJ
一氧化氮(NO)是一种中性自由基气体。长期以来，NO分子一直被认为是一种环境污染物，并对大气中的健康构成潜在危害。直到最近，NO在生物系统中的有益作用才被发现。1-3自从在生物学中突破性地发现NO信号以来的几年里，进一步的研究表明，NO是心血管、免疫和神经系统中普遍存在的信使。1，4-12尽管NO是一个相对稳定的物种，据报道在5秒、5秒、13秒、14秒的生理条件下半衰期长达5秒，但它很容易与生物中常见的各种物种发生反应。NO的靶标包括氧、氧自由基、硫醇、胺和过渡金属离子。NO分别与氧气和超氧化物反应生成活性氮氧化物(RNO)NO2和ONOO−。这两种产物在水环境中都比NO本身更具活性，NO与氧气的反应也可以生成NO2。
Nitric oxide (NO) is a neutral free radical gas. The NO molecule has long been recognized as an environmental contaminant and a potential health hazard in the atmosphere. It was not until recently that beneficial roles for NO were discovered in biological systems.1–3 In the years since the groundbreaking discovery of NO signaling in biology, further work has shown that NO is a ubiquitous messenger in the cardiovascular, immune, and nervous systems.1,4–12 Even though NO is a relatively stable species with reported half-lives under physiological conditions of up to five seconds,5, 13, 14 it readily reacts with a variety of species commonly found in living organisms. The targets of NO include dioxygen, oxygen radicals, thiols, amines, and transition metal ions. The reactions of NO with dioxygen and superoxide result in formation of reactive nitrogen oxide species (RNOS) NO2and ONOO−, respectively. Both products are more reactive than NO itself In aqueous environments, the reactions of NO with dioxygen can also yield NO2.