Heparanase gene expression and its correlation with spontaneous apoptosis in hepatocytes of cirrhotic liver and carcinoma
Heparanase gene expression and its correlation with spontaneous apoptosis in hepatocytes of cirrhotic liver and carcinoma
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DOI：
10.1016/s0959-8049(02)00558-0
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发表时间：
2003-01-01
影响因子：
8.4
通讯作者：
Kaibara, N
中科院分区：
文献类型：
--
作者：
Ikeguchi, M;Hirooka, Y;Kaibara, N
乙酰肝素酶（hep）降解硫酸乙酰肝素蛋白聚糖（HSPG），其是细胞外基质的主要组分。这一过程被认为是肿瘤侵袭或转移的第一步。然而，HSPGs在信号转导中起重要作用。因此，通过hep降解HSPG可以抑制肿瘤细胞生长。在本研究中，我们研究了增强的hep mRNA表达的临床病理意义在48肝细胞癌（HCC）和48非癌肝样本从相同的患者通过定量实时逆转录聚合酶链反应（RT-PCR）。通过免疫组织化学评价肝细胞中的自发性凋亡。相对hep mRNA表达水平被描述为hep/甘油醛-3-磷酸脱氢酶（GAPDH）比率。肝癌组织中hep mRNA水平显著低于非癌组织（P
Heparanase (hep) degrades heparan sulphate proteoglycans, (HSPGs), which are the main components of the extracellular matrix. This process has been considered as the first step of tumour invasion or metastasis. However, HSPGs play an important role in signal transduction. Thus, the degradation of HSPGs by hep may suppress tumour cell growth. In the present study, we investigated the clinicopathological importance of enhanced hep mRNA expression in 48 hepatocellular carcinomas (HCCs) and in 48 non-cancerous liver samples obtained from the same patients by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). Spontaneous apoptosis in the hepatocytes was evaluated by immunohistochemistry. The relative hep mRNA expression levels were described as hep/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ratios. The hep mRNA levels of HCCs were significantly lower than those of non-cancerous livers (P