Ethylene sensing and gene activation in Botrytis cinerea:: A missing link in ethylene regulation of fungus-plant interactions?
Ethylene sensing and gene activation in Botrytis cinerea:: A missing link in ethylene regulation of fungus-plant interactions?
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DOI：
10.1094/mpmi-19-0033
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Sharon, A
中科院分区：
文献类型：
--
作者：
Chagué, V;Danit, LV;Sharon, A
受感染植物产生乙烯是导致植物防御途径激活的早期抗性反应。然而，植物病原体也能够产生乙烯，乙烯不仅对植物而且对病原体也有影响。因此，乙烯可能通过影响植物以及病原体在真菌-植物相互作用中发挥双重作用。为了解决这个问题，我们研究了乙烯对灰霉菌灰葡萄孢的影响，以及它对本氏烟草植株的致病作用。B的暴露。灰霉病菌对乙烯的反应抑制了菌丝体的生长，并引起了大量真菌基因的转录变化。真菌信号突变体的筛选揭示了一个G α无效突变体（Delta bcg 1），它是乙烯不敏感的，在体外过量产生乙烯，并显示出相当大的转录变化，响应乙烯与野生型相比。氨基乙氧基乙烯基甘氨酸（AVG）处理，乙烯非生产N。benthamiana植物比产生乙烯的植物产生大得多的坏死，而向AVG处理的叶中添加乙烯限制了疾病传播。乙烯还影响植物中真菌基因的表达。一个假定的致病性真菌基因，bcspl 1，在乙烯生产植物接种后24小时，但只有48小时后，在乙烯非生产植物接种后的表达增强。结果表明，B.乙烯诱导的植物抗性可能涉及植物乙烯对植物和真菌的影响。
Ethylene production by infected plants is an early resistance response leading to activation of plant defense pathways. However, plant pathogens also are capable of producing ethylene, and ethylene might have an effect not only on the plant but on the pathogen as well. Therefore, ethylene may play a dual role in fungus-plant interactions by affecting the plant as well as the pathogen. To address this question, we studied the effects of ethylene on the gray mold fungus Botrytis cinerea and the disease it causes on Nicotiana benthamiana plants. Exposure of B. cinerea to ethylene inhibited mycellium growth in vitro and caused transcriptional changes in a large number of fungal genes. A screen of fungal signaling mutants revealed a G alpha null mutant (Delta bcg1) which was ethylene insensitive, overproduced ethylene in vitro, and showed considerable transcriptional changes in response to ethylene compared with the wild type. Aminoethoxyvinylglycine (AVG)-treated, ethylene-nonproducing N. benthamiana plants developed much larger necroses than ethylene-producing plants, whereas addition of ethylene to AVG-treated leaves restricted disease spreading. Ethylene also affected fungal gene expression in planta. Expression of a putative pathogenicity fungal gene, bcspl1, was enhanced 24 h after inoculation in ethylene-producing plants but only 48 h after inoculation in ethylene-nonproducing plants. Our results show that the responses of B. cinerea to ethylene are partly mediated by a G protein signaling pathway, and that ethylene-induced plant resistance might involve effects of plant ethylene on both the plant and the fungus.