Emerging investigator series: environment-specific auxiliary substrates tailored for effective cometabolic bioremediation of 1,4-dioxane
Emerging investigator series: environment-specific auxiliary substrates tailored for effective cometabolic bioremediation of 1,4-dioxane
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新兴研究者系列：针对 1,4-二恶烷的有效共代谢生物修复而定制的环境特异性辅助底物
DOI：
10.1039/d2ew00524g
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Li, Mengyan
中科院分区：
文献类型：
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作者：
Deng, Daiyong;Pham, Dung Ngoc;Li, Mengyan
共代谢生物修复是处理1，4-二氧杂环己烷（二氧杂环己烷）和其他新兴污染物的趋势，以满足严格的监管目标（例如，<10 μg L−1），因为生物降解活动可以通过补充辅助基质来促进。在这项研究中，我们比较和研究了两种常见的辅助底物，短链烷烃气体（例如，丙烷和丁烷）和伯醇（例如，1-丙醇、1-丁醇和乙醇），用于在不同的环境基质中去除二氧六环，并以Azoarcus sp.DD4作为接种物。在纯培养水平的物理化学表征表明，丙烷和1-丙醇是有利的刺激细胞生长和二氧六环生物降解DD 4。平行的微观世界测定进行了评估DD 4生物强化在不同的微生物群落从五个不同的环境样品，包括浅，深含水层地下水，受污染的河流沉积物，和城市活性污泥回收的兼容性。丙烷是有效的，在维持有效的二氧六环去除和DD 4在所有环境矩阵的主导地位。值得注意的是，修正案与1-丙醇促进上级二氧六环降解在深层含水层地下水，其中低预处理生物量和处理后的多样性进行了观察，这表明其潜在的内在领域的应用。微生物群落分析和差异排序的结合确定，Ochrobactrum和其他几个土著细菌接种DD 4的增强，暗示它们的共生或互利关系。总的来说，丙烷和1-丙醇可以是为原位生物强化量身定制的有效辅助基质替代品，其有效性受到环境微生物组的密度和结构的影响。
Cometabolic bioremediation is trending for the treatment of 1,4-dioxane (dioxane) and other emerging contaminants to meet stringent regulatory goals (e.g., <10 μg L−1) since biodegradation activities can be fueled by the supplementation of auxiliary substrates. In this study, we compared and investigated the effectiveness of two types of common auxiliary substrates, short-chain alkane gases (e.g., propane and butane) and primary alcohols (e.g., 1-propanol, 1-butanol, and ethanol), for dioxane removal in diverse environmental matrices with Azoarcus sp. DD4 as the inoculum. Physicochemical characterization at the pure culture level revealed that propane and 1-propanol are advantageous for stimulating cell growth and dioxane biodegradation by DD4. Parallel microcosm assays were conducted to assess the compatibility of DD4 bioaugmentation in diverse microbiomes recovered from five different environmental samples, including shallow and deep aquifer groundwater, contaminated river sediment, and municipal activated sludge. Propane was effective in sustaining efficient dioxane removal and the dominance of DD4 across all environmental matrices. Notably, amendment with 1-propanol promoted superior dioxane degradation in the deep aquifer groundwater, in which low pre-treatment biomass and post-treatment diversity were observed, suggesting its potential for intrinsic field applications. The combination of microbial community analysis and differential ranking identified that Ochrobactrum and several other indigenous bacteria were boosted by the inoculation of DD4, implying their commensal or mutualistic relationship. Collectively, propane and 1-propanol can be effective auxiliary substrate alternatives tailored for in situ bioaugmentation and their effectiveness is affected by the density and structure of environmental microbiomes.