Wastewater sludge as a substrate for growth and carrier for rhizobia:: the effect of storage conditions on survival of Sinorhizobium meliloti
Wastewater sludge as a substrate for growth and carrier for rhizobia:: the effect of storage conditions on survival of Sinorhizobium meliloti
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DOI：
10.1016/s0960-8524(01)00202-4
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发表时间：
2002-06-01
影响因子：
11.4
通讯作者：
Prévost, D
中科院分区：
文献类型：
--
作者：
Ben Rebah, F;Tyagi, RD;Prévost, D
用根瘤菌接种豆科植物可以最大限度地固氮并提高植物产量，而无需使用氮肥，因此开发和优化了许多接种剂类型。在我们的研究中，确定了生长培养基、载体、温度和储存期对中华根瘤菌存活的影响。来自 Communaute Urbaine de Quebec 废水处理厂的二次污泥和标准培养基 (YMB) 用于根瘤菌生长。来自 Jonquiere 废水处理厂的脱水污泥、泥炭以及泥炭和污泥的混合物被用作载体材料。结果表明，与标准培养基相比，废水污泥在-20℃冻融过程中对根瘤菌的生存提供了更好的保护。总的来说，结果还表明使用污泥作为载体的适合性，因为它具有与泥炭相同或更高的支持苜蓿中华根瘤菌生存的潜力。对于 YMB 生长的根瘤菌，泥炭基和污泥基载体在 4 和 25 摄氏度储存期间的存活率似乎相似。对于二次污泥生长的根瘤菌来说，在污泥中的存活率比在泥炭基载体中的存活率更好。一般来说，两种载体（污泥和泥炭）中的细胞计数在 4 和 25°C 下可保持高于 10(8) 个细胞/克长达 80 天。然而，对于在 4 ℃ 下储存在泥炭基载体中的二次污泥生长的细胞，在储存第 81 天时，活细胞减少到 10(8) 个细胞/克以下，但与标准（10(7) 个细胞/克载体）相比仍然可以接受。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
The inoculation of legumes with rhizobia is used to maximise nitrogen fixation and enhance the plant yield without using N fertilisers, For this reason many inoculant types were developed and optimised. In our study, the effects of the growth medium, the carrier, the temperature and the storage period were determined on the survival of Sinorhizobium meloliti. Secondary sludge from Communaute Urbaine de Quebec wastewater treatment plant and standard medium (YMB) were used for rhizobial growth. Dehydrated sludge from Jonquiere wastewater treatment plant, peat and a mixture of peat and sludge were used as carrier materials. Results showed that the wastewater sludge offered better protection for rhizobia survival during freezing and thawing at -20 degreesC than the standard medium. In general, results also showed the suitability of using sludge as a carrier because it had the same or a higher potential than peat to support survival of S. meliloti. In the case of YMB-grown rhizobia, peat- and sludge-based carriers appeared to be similar in terms of survival rate during the storage at 4 and 25 degreesC. For secondary sludge-grown rhizobia, the survival was better in sludge than in peat based carrier. Generally, the cell count remained higher than 10(8) cells/g for up to 80 days at 4 and 25 degreesC in both carriers (Sludge and peat). However, for the secondary sludge-grown cells stored in peat-based carrier at 4 degreesC, the viable cells decreased under 10(8) cells/g at the 81st day of storage but remained acceptable compared to the standard (10(7) cells/g of carrier). (C) 2002 Elsevier Science Ltd. All rights reserved.