Defining the Genome Features of Escherichia albertii, an Emerging Enteropathogen Closely Related to Escherichia coli.
Defining the Genome Features of Escherichia albertii, an Emerging Enteropathogen Closely Related to Escherichia coli.
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DOI：
10.1093/gbe/evv211
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发表时间：
2015-11-03
影响因子：
3.3
通讯作者：
Hayashi T
中科院分区：
文献类型：
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作者：
Ooka T;Ogura Y;Katsura K;Seto K;Kobayashi H;Kawano K;Tokuoka E;Furukawa M;Harada S;Yoshino S;Seto J;Ikeda T;Yamaguchi K;Murase K;Gotoh Y;Imuta N;Nishi J;Gomes TA;Beutin L;Hayashi T
阿尔伯蒂埃希菌是最近发现的大肠埃希菌的近亲。这种新兴的肠病原体具有由肠细胞消失位点编码的III型分泌系统（T3SS），类似于肠致病性和肠出血性大肠杆菌（EPEC和EHEC）。还发现了产志贺毒素的菌株。阿尔伯蒂埃希氏杆菌的基因组特征，特别是与其他埃希氏菌的差异，尚未得到很好的澄清。在此，我们对从多个来源分离的29株E. albertii菌株（3个完整序列和26个草图序列）进行了基因组测序，并进行了种内和种内基因组比较。阿尔伯蒂大肠杆菌的基因组大小在4.5 ~ 5.1 Mb之间，小于大肠杆菌。种内基因组比较鉴定了5个种群。结果表明，艾伯蒂埃氏杆菌可能的核心基因组包含3250个基因，而埃希氏菌可能的核心基因组包含1345个基因。我们的分析进一步揭示了E. albertii的几个独特或显著的遗传特征，包括那些已知的生化特征和毒力因子，以及在大肠杆菌中失活的可能活跃的第二T3SS，称为ETT2 （E. coli T3SS 2）。虽然这种生物在体外已被观察到不具有运动能力，但鞭毛生物合成的基因是完全保守的；趋化相关基因已被选择性删除。基于这些结果，我们开发了一套套式聚合酶链反应系统来直接检测阿尔伯氏杆菌。我们的数据定义了阿尔伯蒂大肠杆菌的基因组特征，并为未来研究这一重要的新兴肠道病原体提供了有价值的基础。
Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences from other Escherichia species, have not yet been well clarified. Here, we sequenced the genome of 29 E. albertii strains (3 complete and 26 draft sequences) isolated from multiple sources and performed intraspecies and intragenus genomic comparisons. The sizes of the E. albertii genomes range from 4.5 to 5.1 Mb, smaller than those of E. coli strains. Intraspecies genomic comparisons identified five phylogroups of E. albertii. Intragenus genomic comparison revealed that the possible core genome of E. albertii comprises 3,250 genes, whereas that of the genus Escherichia comprises 1,345 genes. Our analysis further revealed several unique or notable genetic features of E. albertii, including those responsible for known biochemical features and virulence factors and a possibly active second T3SS known as ETT2 (E. coli T3SS 2) that is inactivated in E. coli. Although this organism has been observed to be nonmotile in vitro, genes for flagellar biosynthesis are fully conserved; chemotaxis-related genes have been selectively deleted. Based on these results, we have developed a nested polymerase chain reaction system to directly detect E. albertii. Our data define the genomic features of E. albertii and provide a valuable basis for future studies of this important emerging enteropathogen.