Investigation of Formation of Bacterial Biofilm upon Dead Siblings
Investigation of Formation of Bacterial Biofilm upon Dead Siblings
复制标题
死亡兄弟姐妹上细菌生物膜形成的调查
DOI：
10.1021/acs.langmuir.8b01962
复制
发表时间：
2019
期刊：
影响因子：
3.9
通讯作者：
Zhang Guangzhao
中科院分区：
文献类型：
--
作者：
Wang Zhi;Gong Xiangjun;Xie Jinhong;Xu Zhenbo;Liu Guangming;Zhang Guangzhao
杀菌剂能有效杀灭细菌；然而，留在表面的死亡细菌细胞是否会影响生物膜的后期生长尚不清楚。本研究利用磁力调制原子力显微镜（MF-AFM）对铜绿假单胞菌（pseudomonas aeruginosa, PAO1）的生物膜进行了培养，并对其进化过程进行了研究。生物膜厚度的时间依赖性表明，死亡兄弟姐妹的沉积可以减缓PAO1生物膜的生长。生长在死菌层上的生物膜比生长在活菌层上的生物膜更柔软，这反映在静态弹性模量(E)和动态刚度（kd）缩放到干扰频率(f) askd=kd,0fγ，其中kd，0是缩放因子，γ是幂律指数。我们揭示了较小的种群，而不是细胞外聚合物质（EPS）的变化在死去的兄弟姐妹的生物膜是负责较软的生物膜。本研究为生物膜的形成提供了更好的理解，从而对抗菌医用材料和防污涂料的设计具有重要意义。
Biocides can effectively kill bacteria; however, whether the dead bacterial cells left on the surface influence the later growth of biofilm is unknown. In this study, we have culturedPseudomonas aeruginosa(PAO1) biofilm on their dead siblings and have investigated their evolution by using magnetic force modulation atomic force microscopy (MF-AFM). The time dependence of the biofilm thickness indicates that the deposited dead siblings can slow down the growth of PAO1 biofilm. The biofilm growing on dead bacteria layers is softer in comparison with those upon alive siblings, as reflected by the static elastic modulus (E) and dynamic stiffness (kd) scaled to the disturbing frequency (f) askd=kd,0fγ, wherekd,0is the scaling factor and γ is the power-law exponent. We reveal that the smaller population instead of the variation of extracellular polymeric substances (EPS) within the biofilm upon the dead siblings is responsible for the softer biofilm. The present study provides a better understanding of the biofilm formation, thus, making it significant for designing antimicrobial medical materials and antifouling coatings.