Alterations in the genomes of avian sarcoma viruses.
Alterations in the genomes of avian sarcoma viruses.
复制标题
禽肉瘤病毒基因组的改变。
DOI：
10.1016/0042-6822(82)90484-6
复制
发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Taylor,JM
中科院分区：
文献类型：
--
作者：
Mason,WS;Linial,M;Hsu,TW;Eisenman,RN;Townsend,J;Mark,GE;Seal,G;Aldrich,C;Taylor,JM
We describe 12 nonconditional mutants of avian sarcoma virus. Eleven were replication defective and one appeared to retain wild-type functions. Comparison of restriction endonuclease digests of viral DNA revealed that 8 of 11 replication defectives contained large deletions of viral information. A functionally wild-type variant contained an insertion of sequences between theenvandsrcgenes. The deletions appeared to be randomly distributed throughout the replicative genes:gag, pol, andenv. In most, but not all, cases, the phenotype of the deletion mutants could be simply correlated with the location and extent of the deletions. In a few cases, the defects in expression appeared to be more extensive than would be expected from a single point or deletion mutation, suggesting that these mutants contained multiple mutations or a single mutation in a region affecting several viral functions.
