Tunable nanoscale channels in diblock copolymer films for biomolecule organization.
Tunable nanoscale channels in diblock copolymer films for biomolecule organization.
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二嵌段共聚物薄膜中用于生物分子组织的可调节纳米级通道。
DOI：
10.1021/la100985a
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Composto,RussellJ
中科院分区：
文献类型：
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作者：
Park,JungHyun;Sun,Yujie;Goldman,YaleE;Composto,RussellJ
我们描述了一种方法来创建纳米级的，功能化的通道嵌段共聚物膜，并证明其作为模板连接丝状肌动蛋白（F-肌动蛋白）。表面上的形貌和化学图案分别通过暴露于UV臭氧（UVO）和与胺封端的硅烷反应来产生和控制。连续的UVO曝光降解聚合物域的自催化反应，因此，膜厚度以S形的方式减少。利用每个域的差分蚀刻速率，通过改变UVO曝光时间和嵌段共聚物分子量，分别创建具有可调深度和宽度的纳米级通道。对于垂直层状形态的聚（苯乙烯-b-甲基丙烯酸甲酯），P（S-b-MMA），膜（65 nm），最初表现出较高的MMA域，经历了3分钟的UVO后的高度反转，因为MMA域的蚀刻速度为S域的两倍。在紫外光照射10 min后，晶畴之间的最大高度差为16 nm.对于平行圆柱形态的UVO蚀刻观察到类似的行为。紫外光照射也会在聚苯乙烯和聚甲基丙烯酸甲酯的表面以及P（S-b-MMA）中相应的结构域上产生反应性极性基团。通过将UV处理的膜暴露于3-氨丙基三乙氧基硅烷（APTES），P（S-b-MMA）表面变得富含胺基，其充当生物分子的结合位点。在生理条件下（pH = 7.4），这些带正电荷的纳米结构通过静电相互作用吸引带负电荷的F-肌动蛋白。
We describe an approach to create nanoscale, functionalized channels in block copolymer films and demonstrate their use as templates for attaching filamentous actin (F-actin). Topographic and chemical patterns on the surface are created and controlled by exposure to UV-ozone (UVO) and reacting with an amine-terminated silane, respectively. Continuous UVO exposure degrades polymer domains by an autocatalytic reaction, and thus, film thickness decreases in a sigmoidal manner. Utilizing the differential etching rates of each domain, nanoscale channels with tunable depth and width are created by varying UVO exposure time and block copolymer molecular weight, respectively. For a perpendicular lamellar morphology poly(styrene-b-methyl methacrylate), P(S-b-MMA), films (65 nm), initially exhibiting higher MMA domains, undergo a height inversion after 3 min of UVO because MMA domains etch twice as fast as S domains. The maximum height difference between domains is ∼16 nm after ∼10 min of UVO. Similar behavior is observed for UVO etching of a parallel cylinder morphology. UVO exposure also produces reactive polar groups on the surfaces of poly(styrene) and poly(methyl methacrylate) as well as their corresponding domains in P(S-b-MMA). By exposing UVO-treated films to 3-aminopropyltriethoxysilane (APTES), P(S-b-MMA) surface becomes enriched with amine groups which act as binding sites for biomolecules. Under physiological conditions (pH ∼ 7.4), these positively charged nanostructures attract negatively charged F-actin by an electrostatic interaction.