STUDIES ON THE NUTRITION OF PASTEURELLA TULARENSIS
STUDIES ON THE NUTRITION OF PASTEURELLA TULARENSIS
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土拉巴氏杆菌的营养研究
DOI：
10.1128/jb.70.1.60-69.1955
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发表时间：
1955
影响因子：
3.2
通讯作者：
N. Grossowicz
中科院分区：
文献类型：
--
作者：
A. Traub;J. Mager;N. Grossowicz
Since Francis' (1922) discovery that Pateurella tularensis can be cultured on blood-peptone agar enriched with cysteine, subsequent work (Ransmeier and Schaub, 1941; Ransmeier and Stekol, 1942; Steinhaus, Parker, and McKee, 1944; Larson, 1945) reemphasized the high demand for cysteine or cystine as the most characteristic nutritional feature of this microorganism. The study of Berkman (1942) appears to be the first attempt aimed at the elucidation of the basic nutritional requirements of P. tularensis. Using a gelatin hydrolyzate fortified with several amino acids as a basal medium he demonstrated the need for thiamin or cocarboxylase as an essential growth factor. Tamura and Gibby (1943) and Gibby et al. (1948) were able to grow P. tularensis in liquid media composed of protein hydrolyzates or an amino acid mixture supplemented with an extract of blood cells. Mills et al. (1949) in a brief communication reported that good growth of a highly virulent strain of Bacterium tularense was obtained in a medium containing casein hydrolyzate, glucose, cysteine, salts, and 0.1 per cent decamin (a dried yeast autolyzate). The casein hydrolyzate could be replaced by a mixture of amino acids. Tamura and Fleming (1949) in a preliminary report announced the cultivation of B. tularense in a synthetic medium with cocarboxylase, adenylic acid, adenosine diphosphate, or adenosine triphosphate as the critical growth factors. No experimental data were presented. With the strains at our disposal we were unable to obtain growth in the semisynthetic or synthetic media described in the literature. In a preliminary communication (Mager, Traub, and Grossowicz, 1954) the factors determining growth of P. tularensis in chemically defined media have been discussed. In the present paper results of a
