Müller cell expression of glial fibrillary acidic protein after genetic and experimental photoreceptor degeneration in the rat retina.
Müller cell expression of glial fibrillary acidic protein after genetic and experimental photoreceptor degeneration in the rat retina.
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发表时间：
1984-11
影响因子：
4.4
通讯作者：
A. Eisenfeld;Ann H. Bunr-Milam;P. V. Sarrhy
中科院分区：
文献类型：
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作者：
A. Eisenfeld;Ann H. Bunr-Milam;P. V. Sarrhy
胶质纤维酸性蛋白(GFAP)通常存在于星形胶质细胞中。在各年龄段正常大鼠视网膜中，只有星形胶质细胞表达GFAP。这种染色模式也在小于38天的遗传性视网膜营养不良的RCS大鼠中发现。从第38天开始，当大约61%的光感受器退化时，少数GFAP阳性纤维横跨视网膜从内界膜到外界膜。到第41天和以后的所有年龄，米勒细胞的放射状纤维是对光感受器坏死的反应或可能是突变基因的直接影响。我们通过不同时间的恒定光照诱导正常成年SD大鼠的光感受器退化。恒光照射3d后，光感受器数量减少20%，许多Müler细胞GFAP阳性。免疫印迹研究证实，抗GFAP与来自视网膜的单一蛋白质反应，该蛋白质的相对分子质量和Triton不溶性与GFAP相对应。免疫印迹也证实了对照组和实验视网膜抗GFAP免疫染色的结果。这些结果表明，在实验和遗传诱导的光感受器变性反应中，Müler细胞表达GFAP免疫反应。
Glial fibrillary acidic protein (GFAP) is normally found in astrocytes. In the normal rat retina at all ages, only astrocytes stain for GFAP. This staining pattern is also found in RCS rats with inherited retinal dystrophy younger than 38 days. Beginning on day 38, when about 61% of the photoreceptors have degenerated, a few GFAP-positive fibers span the retina from the inner limiting membrane to the external limiting membrane. By day 41 and at all later ages examined, the radial fibers of Müller cells is a response to photoreceptor necrosis or might be a direct effect of the mutant gene, we induced photoreceptor degeneration in normal, adult Sprague-Dawley rats by exposing them to constant light for variable periods of time. After 3 days in constant light, there is a 20% reduction in the number of photoreceptors and many Müller cells are positive for GFAP. Immunoblot studies confirmed that the anti-GFAP reacted with a single protein from retina that corresponded in molecular weight and Triton-insolubility to GFAP. The immunoblots also corroborated the results from anti-GFAP immunostaining of control and experimental retinas. These results indicate that Müller cells express GFAP immunoreactivity in response to experimentally as well as genetically induced photoreceptor degeneration.