Enhanced Suppressor Cell Activity as a Mechanism of Immunosuppression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin 1
Enhanced Suppressor Cell Activity as a Mechanism of Immunosuppression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin 1
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2,3,7,8-四氯二苯并-对二恶英 1 增强抑制细胞活性作为免疫抑制机制
DOI：
10.3181/00379727-168-41275
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发表时间：
1981
影响因子：
--
通讯作者：
G. Sweeney
中科院分区：
文献类型：
--
作者：
D. Clark;J. Gauldie;M. Szewczuk;G. Sweeney
摘要TCDD（2，3，7，8-四氯二苯并对二恶英）是一种有毒的卤代芳烃，在人体内具有蓄积性毒性。用C57 B1/6雄性小鼠研究了TCDD的慢性免疫毒性作用。100 μg/kg或更高总剂量导致胸腺、脾脏和淋巴结细胞耗竭，动物出现疾病。在0.4 μg/kg剂量下未观察到细胞耗竭，仅胸腺受到4和40 μg/kg剂量的影响。40μg/kg TCDD可降低小鼠对SRBC和TNP-流产布鲁氏菌的抗体反应，4 μg/kg TCDD可降低小鼠对恶唑酮的迟发型超敏反应，而同种抗原特异性细胞毒性T细胞（CTL）的产生仅对0.004 μg/kg TCDD敏感。在体外的抑制机制的分析，使用有限稀释技术表明，TCDD没有耗尽的前体细胞的CTL，但产生的细胞能够抑制CTL的产生在体外。
Abstract TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin), a toxic halogenated aromatic hydrocarbon, acts in the body as a cumulative poison. The chronic immunotoxic effects of TCDD were studied in C57B1/6 male mice. Total doses of 100 μg/kg or greater produced cellular depletion in thymus, spleen, and lymph nodes, and the animals appeared sick. No cellular depletion was seen following 0.4 μg/kg, and only thymus was affected by 4 and 40 μg/kg. The antibody response to SRBC and TNP-Brucella abortus was impaired following 40μg/kg TCDD, the delayed hypersensitivity response to oxazalone was impaired by 4 μg/kg and the generation of alloantigen-specific cytotoxic T cells (CTL) was sensitive to as little as 0.004 μg/kg TCDD. In vitro analysis of the mechanism of suppression using limiting dilution techniques showed that TCDD did not deplete the precursors of CTL but generated cells capable of suppressing CTL generation in vitro.