Doxycycline-coated sutures improve the suture-holding capacity of the rat Achilles tendon
Doxycycline-coated sutures improve the suture-holding capacity of the rat Achilles tendon
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DOI：
10.1080/17453670710014392
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发表时间：
2007-01
影响因子：
3.7
通讯作者：
B. Pasternak;Anna Missios;A. Askendal;P. Tengvall;P. Aspenberg
中科院分区：
文献类型：
--
作者：
B. Pasternak;Anna Missios;A. Askendal;P. Tengvall;P. Aspenberg
背景：有证据表明肌腱缝合处周围存在高基质金属蛋白酶（MMP）活性。这可能导致组织破裂，使缝线穿过肌腱，从而导致修复部位伸长和间隙形成。因此，我们假设用MMP抑制剂强力霉素治疗可以改善肌腱的缝合能力。动物、方法和结果在第一个子研究中，大鼠的跟腱接受了缝合。一组患者接受全身性强力霉素治疗，另一组患者未接受任何治疗。在第3、5和7天，机械测量缝线保持能力。与新插入的缝线相比，所有肌腱的拔出力和能量在3天时均降低，但在随后的时间点未检测到进一步降低。强力霉素处理的肌腱显示出改善的缝合保持能力，通过测量比未处理的肌腱更高的能量吸收。破坏力显示出改善的趋势。效果在第3天最明显。在第二子研究中，缝线涂有强力霉素。在第3天，局部强力霉素治疗引起了缝线保持能力的改善，这是通过更高的失败力和更高的能量吸收来测量的。解释我们提供了一个新的治疗原则的证据。MMP支架涂层缝线可改善胶原组织早期修复过程中的缝线固定能力。
Background There is evidence of high matrix metalloproteinase (MMP) activity around sutures inserted into tendons. This probably results in tissue breakdown, allowing the suture to cut through the tendon, and thus contributes to repair-site elongation and gap formation. We therefore hypothesized that treatment with the MMP inhibitor doxycycline would improve the sutureholding capacity of tendon. Animals, methods and results In the first sub-study, rats received a suture in the Achilles tendon. One group was treated with systemic doxycycline and the other received no treatment. At 3, 5, and 7 days, suture-holding capacity was measured mechanically. The pull-out force and energy were reduced in all tendons, at 3 days compared to freshly inserted sutures, but no further reduction was detected at later time points. Doxycycline- treated tendons showed improved suture-holding capacity as measured by higher energy uptake than in untreated tendons. Force at failure showed a trend towards improvement. The effect was most evident on day 3. In the second sub-study, sutures were coated with doxycycline. At 3 days, local doxycycline treatment caused improved suture-holding capacity—as measured by higher force at failure and higher energy uptake. Interpretation We provide proof of a novel treatment principle. MMP inhibitor-coated sutures improve suture-holding capacity during early repair of collagenous tissues.