Development of both human connective tissue-type and mucosal-type mast cells in mice from hematopoietic stem cells with identical distribution pattern to human body
Development of both human connective tissue-type and mucosal-type mast cells in mice from hematopoietic stem cells with identical distribution pattern to human body
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DOI：
10.1182/blood-2003-04-1160
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发表时间：
2004-02-01
期刊：
影响因子：
20.3
通讯作者：
Nakahata, T
中科院分区：
文献类型：
--
作者：
Kambe, N;Hiramatsu, H;Nakahata, T
将原始人类细胞移植到亚致死辐射免疫缺陷小鼠体内，是研究人类造血干细胞功能的一种成熟的体内系统。尽管肥大细胞是造血干细胞的后代，但在接受人造血干细胞移植的小鼠中，人类肥大细胞的发育尚未见报道。在这里，我们报道了人类造血干细胞异种移植到患有严重联合免疫缺陷和白细胞介素2 （IL-2）受体γ链等位基因突变的非肥胖糖尿病严重联合免疫缺陷(NOD/SCID)/ γ (c)(null) （NOG）小鼠后的人类肥大细胞发育。在小鼠环境的支持下，人类肥大细胞簇在小鼠真皮中发育，但它们比其他形式的人类细胞重建需要更多的时间。在肺和胃道中，粘膜型肥大细胞含有胰蛋白酶，但缺乏乳糜酶，位于胃粘膜和肺泡中，而结缔组织型肥大细胞含有胰蛋白酶和乳糜酶，位于胃粘膜下层和主要气道周围，如在人体中。在淋巴结、脾脏和腹腔中也观察到肥大细胞的发育，但在外周血中没有。将人造血干细胞异种移植到NOG小鼠体内，有望为研究人肥大细胞的体内发育和功能提供一个高效的模型。(C) 2004年由美国血液病学会出版。
The transplantation of primitive human cells into sublethally irradiated immune-deficient mice is the well-established in vivo system for the investigation of human hematopoietic stem cell function. Although mast cells are the progeny of hematopoietic stem cells, human mast cell development in mice that underwent human hematopoietic stem cell transplantation has not been reported. Here we report on human mast cell development after xenotransplantation of human hematopoietic stem cells into nonobese diabetic severe combined immunodeficient (NOD/SCID)/gamma(c)(null) (NOG) mice with severe combined immunodeficiency and interleukin 2 (IL-2) receptor gamma-chain allelic mutation. Supported by the murine environment, human mast cell clusters developed in mouse dermis, but they required more time than other forms of human cell reconstitution. In lung and gastric tract, mucosal-type mast cells containing tryptase but lacking chymase located on gastric mucosa and in alveoli, whereas connective tissue-type mast cells containing both tryptase and chymase located on gastric submucosa and around major airways, as in the human body. Mast cell development was also observed in lymph nodes, spleen, and peritoneal cavity but not in the peripheral blood. Xenotransplantation of human hematopoietic stem cells into NOG mice can be expected to result in a highly effective model for the investigation of human mast cell development and function in vivo. (C) 2004 by The American Society of Hematology.