Enhancement of phenolic acid content and antioxidant activity of rice bran fermented with Rhizopus oligosporus and Monascus purpureus
Enhancement of phenolic acid content and antioxidant activity of rice bran fermented with Rhizopus oligosporus and Monascus purpureus
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DOI：
10.1016/j.bcab.2014.11.003
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发表时间：
2015-01-01
影响因子：
4
通讯作者：
Long, Kamariah
中科院分区：
文献类型：
--
作者：
Abd Razak, Dang Lelamurni;Abd Rashid, Nur Yuhasliza;Long, Kamariah
研究了几种真菌固态发酵对米糠中酚酸含量和抗氧化活性的影响，并与未发酵的米糠进行了比较。用少孢根霉和紫云杉两种真菌的单一和混合培养物发酵底物。用Folin-Ciocalteu法测定的总酚含量(TPC)在发酵后增加。用血浆铁还原能力(FRAP)法对其抗氧化活性进行了分析，其变化趋势与TPC一致，即所有发酵样品的抗氧化活性均有所提高。与TPC和FRAP法相比，发酵样品对DPPH(2，2-二苯基-1-苦基肼)自由基的清除活性没有显著提高或降低。用高效液相色谱法测定发酵RB的甲醇提取物中的酚酸含量，结果表明，阿魏酸、芥子酸、香草酸、咖啡酸、丁香酸和4-羟基苯甲酸的含量显著增加。本研究的结果证明，利用真菌固态发酵可以提高RB的整体抗氧化活性和酚酸含量。(C)爱思唯尔有限公司出版的2014年。
The effect of solid-state fermentation using several types of fungi on the phenolic acid content and antioxidant activity of rice bran (RB) was determined and compared to the non-fermented counterpart. The substrate was fermented with single and mixed cultures of two types of fungi: Rhizopus oligosporus and Monuscus purpureus. The total phenolic content (TPC), determined using the Folin-Ciocalteu method, increased upon fermentation. The antioxidant activity was analyzed using the ferric reducing ability of plasma (FRAP) method, which showed the same trend as TPC; that is, the antioxidant activity improved in all fermented samples. In contrast to the TPC and FRAP assays, the DPPH (2,2-dipheny1-1-picrylhydrazyl) radical scavenging activity of the fermented samples exhibited either an insignificant improvement or reduced activity. The phenolic acid content in methanol extracts of fermented RB, determined using high performance liquid chromatography (HPLC), generally showed a significant increase in ferulic, sinapic, vanillic, caffeic, syringic, and 4-hydroxybenzoic acids. The results of this study prove that the overall antioxidant activity and phenolic acid content of RB can be enhanced by solid-state fermentation using fungi. (C) 2014 Published by Elsevier Ltd.