A novel approach for freeze-frame video determination of volumetric blood flow in the rat retina.
A novel approach for freeze-frame video determination of volumetric blood flow in the rat retina.
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DOI：
10.3928/1542-8877-20031101-16
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发表时间：
2003-11
期刊：
影响因子：
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通讯作者：
B. Khoobehi;G. Peyman;L. G. Carnahan;R. L. Hayes
中科院分区：
文献类型：
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作者：
B. Khoobehi;G. Peyman;L. G. Carnahan;R. L. Hayes
背景与目的本研究的目的是利用定格技术定量视网膜血流动力学，以确定体积视网膜血流。材料和方法从大鼠循环中取出白细胞和红细胞，用荧光染料染色，返回动物循环，并使用扫描激光检眼镜激发荧光。对视网膜循环进行录像并逐帧观察，以计数在已知时间段内通过视野的可见染色细胞的总数，同时排除多帧中细胞的重复出现。可视化的细胞总数，结合在同时的体外研究中收集的细胞密度数据，用于计算两种细胞类型的体内体积血流量。结果：基于荧光白细胞（34.2 +/- 5.9 nL/sec [平均值+/-标准差]; n = 30只大鼠）和荧光红细胞（35.1 +/- 6.1 nL/sec; n = 30只大鼠）的血流量体积测量值无显著差异，表明定格技术提供了一种与细胞类型无关的血流量测量。随着时间的推移，从单个大鼠获得的重复血流量测量结果几乎没有差异（P < .0001），证明了该技术的可重复性。结论：冻结帧技术提供了一种很有前途的方法来计算体积血流量独立于单个细胞的速度，大小，或类型。
BACKGROUND AND OBJECTIVE The goal of this study was to quantify retinal hemodynamics using the freeze-frame technique for determining volumetric retinal blood flow. MATERIALS AND METHODS Leukocytes and erythrocytes were removed from the circulation of rats, stained with fluorescent dyes, returned to the animals' circulation, and excited to fluorescence using the scanning laser ophthalmoscope. The retinal circulation was videotaped and viewed frame-by-frame to count the total number of visible stained cells passing through the field of view during a known period of time while excluding repeated appearances of cells in multiple frames. The total number of cells visualized, in conjunction with cell density data collected in a coincident in vitro study, was used to calculate in vivo volumetric blood flow for both cell types. RESULTS Measurements of volumetric blood flow based on fluorescent leukocytes (34.2 +/- 5.9 nL/sec [mean +/- standard deviation]; n = 30 rats) and fluorescent erythrocytes (35.1 +/- 6.1 nL/sec; n = 30 rats) were not significantly different, indicating that the freeze-frame technique provides a blood flow measurement independent of cell type. Repeated blood flow measurements obtained from an individual rat over time showed little difference (P < .0001), demonstrating the reproducibility of the technique. CONCLUSIONS The freeze-frame technique offers a promising method for calculating volumetric blood flow independent of individual cell velocity, size, or type.