The TERB1-TERB2-MAJIN complex of mouse meiotic telomeres dates back to the common ancestor of metazoans
The TERB1-TERB2-MAJIN complex of mouse meiotic telomeres dates back to the common ancestor of metazoans
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DOI：
10.1186/s12862-020-01612-9
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发表时间：
2020-05-14
影响因子：
3.4
通讯作者：
Benavente, Ricardo
中科院分区：
文献类型：
--
作者：
da Cruz, Irene;Brochier-Armanet, Celine;Benavente, Ricardo
减数分裂是有性生殖所必需的，从二倍体生殖细胞产生遗传多样性的单倍体配子。在减数分裂前期1，染色体的运动需要端粒附着在核膜上。这种附着是由端粒连接蛋白介导的。迄今为止，已经在裂变酵母和小鼠中发现了端粒衔接蛋白。在小鼠体内，它们形成由减数分裂蛋白TERB1、TERB2和MAJIN组成的复合体。这三种小鼠蛋白与裂变酵母的接头蛋白之间没有序列相似性，这就提出了这个特定蛋白复合物的进化史和意义的问题。结果TERB1、TERB2和MAJIN蛋白存在于整个后生动物中，甚至存在于刺胞菌、Placozoa和Porifera等早期分支的非双侧门中。后生动物TERB1、TERB2和MAJIN在所有进化枝上都显示出类似的域结构。此外，参与复合物形成的蛋白质结构域以及与端粒庇护蛋白和LINC复合物相互作用的蛋白质结构域显示出高度的序列相似性。最后，刺胞动物vulgaris的基因表达证明了TERB1-TERB2-MAJIN复合物在种系中是选择性表达的。结论TERB1-TERB2-MAJIN复合体在后生动物中具有古老的起源，表明其减数分裂功能的保存。
Background Meiosis is essential for sexual reproduction and generates genetically diverse haploid gametes from a diploid germ cell. Reduction of ploidy depends on active chromosome movements during early meiotic prophase I. Chromosome movements require telomere attachment to the nuclear envelope. This attachment is mediated by telomere adaptor proteins. Telomere adaptor proteins have to date been identified in fission yeast and mice. In the mouse, they form a complex composed of the meiotic proteins TERB1, TERB2, and MAJIN. No sequence similarity was observed between these three mouse proteins and the adaptor proteins of fission yeast, raising the question of the evolutionary history and significance of this specific protein complex. Result Here, we show the TERB1, TERB2, and MAJIN proteins are found throughout the Metazoa and even in early-branching non-bilateral phyla such as Cnidaria, Placozoa and Porifera. Metazoan TERB1, TERB2, and MAJIN showed comparable domain architecture across all clades. Furthermore, the protein domains involved in the formation of the complex as well as those involved for the interaction with the telomere shelterin protein and the LINC complexes revealed high sequence similarity. Finally, gene expression in the cnidarian Hydra vulgaris provided evidence that the TERB1-TERB2-MAJIN complex is selectively expressed in the germ line. Conclusion Our results indicate that the TERB1-TERB2-MAJIN complex has an ancient origin in metazoans, suggesting conservation of meiotic functions.