Expression of conserved mucin domains by epithelial tissues in various mammalian species
Expression of conserved mucin domains by epithelial tissues in various mammalian species
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DOI：
10.1016/j.rvsc.2008.05.011
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发表时间：
2009-02-01
影响因子：
2.4
通讯作者：
Croce, Maria V.
中科院分区：
文献类型：
--
作者：
Lacunza, Ezequiel;Bara, Jacques;Croce, Maria V.
粘蛋白与兽医和人类医学中的传染性和非传染性疾病有关。 MUC1粘蛋白是在人上皮细胞顶端表面表达的跨膜糖蛋白，而MUC5AC是在人胃上皮以及肺和眼的杯状细胞中表达的主要分泌粘蛋白。 MUC5AC C 端富含半胱氨酸的区域和 MUC1 结构域的胞质尾部在多种哺乳动物物种中是保守的。目的：比较哺乳动物上皮细胞中MUC1和MUC5AC粘蛋白的表达。使用CT33抗MUC1胞质尾(MUC1CT)多克隆抗体和45M1抗MUC5AC单克隆抗体。免疫组织化学显示，MUC1CT 在大多数组织中表达，而 MUC5AC 仅限于胃表面上皮以及气管和肺的杯状细胞。通过蛋白质印迹，MUC1CT 在大多数组织中显示出约 35 kDa 的条带； MUC5AC 在大鼠、猫、猪和牛的胃和肺分泌物中显示出 > 180 kDa 的条带。当对大鼠MUC5AC进行免疫沉淀时，获得约180kDa的条带。 (c) 2008 Elsevier Ltd. 保留所有权利。
Mucins are related to infectious and non-infectious diseases in Veterinary and Human Medicine. MUC1 mucin is a transmembrane glycoprotein expressed on the apical surface of human epithelia while MUC5AC is the predominant secreted mucin expressed in human gastric epithelium and goblet cells of lung and eyes. MUC5AC C-terminus cysteine rich regions and the cytoplasmic tail of MUC1 domains are conserved among several mammalian species. Objective: to compare the expression of MUC1 and MUC5AC mucins in mammalian epithelia. CT33 anti-MUC1 cytoplasmic tail (MUC1CT) polyclonal antibody and 45M1 anti-MUC5AC monoclonal antibody were employed. By immunohistochemistry, MUC1CT was expressed in most tissues while MUC5AC was restricted to gastric surface epithelium and goblet cells from trachea and lung. By western blot, MUC1CT showed a band at approximately 35 kDa in most tissues; MUC5AC revealed bands at > 180 kDa in stomach and lung secretions from rat, cat, pig and cow. When rat MUC5AC was immunoprecipitated, a band at about 180 kDa was obtained. (c) 2008 Elsevier Ltd. All rights reserved.