Disseminated intravascular coagulation (DIC). Immunohistochemical study of fibrin-related materials (FRMs) in renal tissues.
Disseminated intravascular coagulation (DIC). Immunohistochemical study of fibrin-related materials (FRMs) in renal tissues.
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弥散性血管内凝血（DIC）。
DOI：
10.1111/j.1440-1827.1987.tb03135.x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Michiyasu Awai
中科院分区：
文献类型：
--
作者：
Hideaki Kawasaki;Keiki Hayashi;Michiyasu Awai
对208例尸检患者的肾组织进行了检查。恶性肿瘤合并血液系统恶性肿瘤常合并DIC。组织切片苏木精-伊红染色和Mallory氏磷钨酸苏木精(PtaH)染色，用抗人纤维蛋白原、FDP-D和FDP-E的血清进行免疫过氧化物酶染色(IP)。组织学上，80例(38%)肾小球毛细血管和/或肾小管内可见纤维蛋白或纤维蛋白原相关物质。在26例临床诊断为DIC的患者中，有23例(88%)FRMS呈PtaH或IP阳性或两者同时阳性。在其余3例中，抗凝药物可能干扰了FRMS的观察。这项研究表明，Ptah染色是非特异性的，对FRMS不敏感，IP对于DIC的病理诊断是必要的。肾小管FRMS的存在是确认DIC的一个重要发现。DIC可能在没有临床DIC症状的情况下出现在组织学上。
Renal tissues of 208 autopsied cases were examined. Malignant neoplasm with hematological malignancies often accompanied DIC. Tissue sections were stained with hematoxylin and eosin and Mallory's phosphotungstic acid hematoxylin (PTAH), and were applied for immunoperoxidase method (IP), using antisera against human fibrinogen, FDP-D and FDP-E. Histologically in 80 cases (38%) fibrin or fibrinogen related materials (FRMs) were observed in the glomerular capillary or the intratubular area or in both. FRMs were PTAH or IP positive or both in 23 of the 26 cases (88%) clinically diagnosed as DIC. In the remaining three cases anticoagulants probably interfered with FRMs observation. This study showed the PTAH stain was nonspecific and insensitive to FRMs, and that IP was necessary for a pathological diagnosis of DIC. The presence of FRMs in the renal tubuli is an important finding in confirming DIC. DIC may be present histologically in the absence of clinical DIC symptoms.