CD69 gene is differentially regulated in T and B cells by evolutionarily conserved promoter-distal elements.
CD69 gene is differentially regulated in T and B cells by evolutionarily conserved promoter-distal elements.
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DOI：
10.4049/jimmunol.0900839
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发表时间：
2009-11-15
期刊：
影响因子：
--
通讯作者：
Lauzurica P
中科院分区：
文献类型：
--
作者：
Vazquez BN;Laguna T;Carabana J;Krangel MS;Lauzurica P
CD69 is a type II C-type lectin involved in lymphocyte migration and cytokine secretion. CD69 expression represents one of the earliest available indicators of leukocyte activation and its rapid induction occurs through transcriptional activation. In this study we examined the molecular mechanism underlying mouse CD69 gene transcription in vivo in T and B cells. Analysis of the 45kb region upstream of the CD69 gene revealed evolutionary conservation at the promoter and at four non-coding sequences (CNS) that were called CNS1, CNS2, CNS3 and CNS4. These regions were found to be hypersensitive sites in DNase I digestion experiments and chromatin immunoprecipitation assays showed specific epigenetic modifications. CNS2 and CNS4 displayed constitutive and inducible enhancer activity in transient transfection assays in T cells. Using a transgenic approach to test CNS function, we found that the CD69 promoter conferred developmentally regulated expression during positive selection of thymocytes but could not support regulated expression in mature lymphocytes. Inclusion of CNS1 and CNS2 caused suppression of CD69 expression whereas further addition of CNS3 and CNS4 supported developmental-stage and lineage-specific regulation in T cells but not in B cells. We concluded CNS1-4 are important cis-regulatory elements that interact both positively and negatively with the CD69 promoter and that differentially contribute to CD69 expression in T and B cells.
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影响因子：
32.4
作者：
Decker, Thorsten;di Magliano, Marina Pasca;Busslinger, Meinrad
通讯作者：
Busslinger, Meinrad
影响因子：
32.4
作者：
He, Xi;Park, Kyewon;Kappes, Dietmar J.
通讯作者：
Kappes, Dietmar J.
影响因子：
30.5
作者：
Avni, O;Lee, D;Rao, A
通讯作者：
Rao, A
DOI：
10.1084/jem.178.2.537
发表时间：
1993-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
López-Cabrera M;Santis AG;Fernández-Ruiz E;Blacher R;Esch F;Sánchez-Mateos P;Sánchez-Madrid F
通讯作者：
Sánchez-Madrid F
影响因子：
4.4
作者：
Han, Yanmei;Guo, Qiuli;Cao, Xuetao
通讯作者：
Cao, Xuetao