Single-Molecule Arrays for Protein and Nucleic Acid Analysis
Single-Molecule Arrays for Protein and Nucleic Acid Analysis
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DOI：
10.1146/annurev-anchem-061516-045340
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Walt, David R.
中科院分区：
文献类型：
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作者：
Cohen, Limor;Walt, David R.
在过去的几年里，我们已经取得了突破，这将改变我们测量重要分析物的能力。反应体积的小型化和将感兴趣的分析物限制在超小容器中已经极大地提高了许多检测方法的灵敏度和通量。微孔阵列的制造和基于珠粒的测定的实施在用于测量相关生物分子的方法的开发中是有用的，其应用于诊断和基础生物学研究。在这篇综述中，我们描述了如何制造和利用微孔阵列来测量感兴趣的分析物。然后，我们讨论了数字酶联免疫吸附测定（ELISA）的基本概念，使用单分子阵列和微孔阵列的超灵敏蛋白质测量的应用。我们还探讨了微孔阵列用于核酸检测的实用性和单细胞研究的应用。
The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement of analytes of interest into ultrasmall containers have greatly enhanced the sensitivity and throughput of many detection methods. Fabrication of microwell arrays and implementation of bead-based assays have been instrumental in the development of methods for measuring relevant biomolecules, with applications to both diagnostics and fundamental biological studies. In this review, we describe how microwell arrays are fabricated and utilized for measuring analytes of interest. We then discuss the fundamental concepts of digital enzyme-linked immunosorbent assay (ELISA) using single-molecule arrays and applications of microwell arrays to ultrasensitive protein measurements. We also explore the utility of microwell arrays for nucleic acid detection and applications for single-cell studies.