MICROBATCH CRYSTALLIZATION UNDER OIL - A NEW TECHNIQUE ALLOWING MANY SMALL-VOLUME CRYSTALLIZATION TRIALS
MICROBATCH CRYSTALLIZATION UNDER OIL - A NEW TECHNIQUE ALLOWING MANY SMALL-VOLUME CRYSTALLIZATION TRIALS
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DOI：
10.1016/0022-0248(92)90241-a
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发表时间：
1992-08-01
影响因子：
1.8
通讯作者：
BLOW, DM
中科院分区：
文献类型：
--
作者：
CHAYEN, NE;STEWART, PDS;BLOW, DM
描述了一种使用非常小的样品进行蛋白质快速结晶的方法。使用计算机控制的微分配器在油下将结晶样品制备为微批量液滴。将1-2穆尔的样品预先混合并以良好的精密度进行分配。样品受到保护，免受油的蒸发、污染和物理冲击。当使用系统的一种模式发现结晶的有利条件时，通过第二程序优化晶体的尺寸和数量，该第二程序在整个感兴趣的区域产生一组条件。在1穆尔滴中生长出衍射尺寸和质量的晶体。
An approach to rapid protein crystallization using very small samples is described. A computer controlled microdispenser is used to make crystallization samples as microbatch droplets under oil. Samples of 1-2 mul are dispensed ready-mixed and with good precision. The samples are protected from evaporation, contamination and physical shock by the oil. When favourable conditions for crystallization have been found using one mode of the system, the size and quantity of crystals are optimized by a second program which generates a set of conditions throughout the area of interest. Crystals of diffraction size and quality have been grown in 1 mul drops.