Expression of discoidin domain receptor 1 during mouse brain development follows the progress of myelination
Expression of discoidin domain receptor 1 during mouse brain development follows the progress of myelination
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DOI：
10.1016/j.neuroscience.2006.02.033
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Vilella, E.
中科院分区：
文献类型：
--
作者：
Franco-Pons, N.;Virgos, C.;Vilella, E.
盘状结构域受体1是在包括脑在内的多种组织中表达的酪氨酸激酶受体。本研究描述了发育过程中小鼠脑中盘状结构域受体1的mRNA和蛋白表达，并对其在胶质细胞生成和神经发生中的作用提供了新的见解。我们进行了原位杂交discoldin域受体1在小鼠胚胎18天，出生后第5，9，15，21天和成年期的大脑，并观察到在胚胎发育过程中的增殖区的扩散模式。从出生后第5天起，discoldin结构域受体1的细胞表达模式被观察到，主要位于白色物质束和以下的时空模式，重叠的髓鞘形成的进展。接下来，我们进行了双标记反应（原位杂交，随后进行化学分析），证实了盘状结构域受体1由成熟的少突胶质细胞表达。我们观察到盘状结构域受体1阳性的细胞也表达肌肽和抗腺瘤性结肠息肉病，两个成熟的少突胶质细胞标记。基于盘状结构域受体I的本地化，特别是在出生后的发展过程中的白色物质纤维束，我们建议，盘状结构域受体I参与髓鞘的发展和维护。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Discoidin domain receptor 1 is a tyrosine kinase receptor expressed in a variety of tissues including the brain. This study describes mRNA and protein expression of discoidin domain receptor 1 in mouse brain during development and provides new insights into its role during gliogenesis and neurogenesis. We performed in situ hybridization for discoldin domain receptor 1 in mouse brains at embryonic day 18, postnatal days 5, 9, 15, 21 and adulthood and observed a diffuse pattern in the proliferative areas during embryogenesis. From postnatal day 5 onwards, a defined cellular expression pattern of discoldin domain receptor 1 was observed, mainly located in white matter tracts and following a spatio-temporal pattern that overlapped the progress of myelination. Next, we performed double-labeling reactions (in situ hybridization followed by immunohistochernistry) that confirmed that discoidin domain receptor 1 was expressed by mature oligodendrocytes. We observed that cells positive for discoidin domain receptor 1 also expressed carnosine and antiadenomatous polyposis coli, two mature oligodendrocyte markers. Based on the localization of discoidin domain receptor I specifically in the white matter fiber tracts during postnatal development, we suggest that discoldin domain receptor I participates in the development and maintenance of the myelin sheath. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.