Inactivation of regulatory volume decrease in human peripheral blood lymphocytes by N-ethylmaleimide.
Inactivation of regulatory volume decrease in human peripheral blood lymphocytes by N-ethylmaleimide.
复制标题
N-乙基马来酰亚胺使人外周血淋巴细胞调节体积减少失活。
DOI：
10.1016/0006-291x(83)91554-1
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Lauf,PK
中科院分区：
文献类型：
--
作者：
Bauer,J;Lauf,PK
发现巯基试剂N-乙基马来酰亚胺以剂量依赖性方式抑制在缓冲低渗氯化钠培养基中肿胀的人外周淋巴细胞的调节性体积减少。在低渗KCl培养基中，NEM处理的淋巴细胞防止了对照淋巴细胞中观察到的额外继发性肿胀。这些数据表明，N-乙基马来酰亚胺作用于涉及体积调节变化的离子转运机制。这种效应与某些哺乳动物红细胞中N-乙基马来酰亚胺明显体积敏感性KCl通量的刺激形成对比。
The sulfhydryl group reagent N-ethylmaleimide was found to inhibit in a dose dependent manner regulatory volume decrease of human peripheral lymphocytes swollen in buffered hyposmotic NaCl media. In hyposmotic KCl media NEM treated lymphocytes prevented an additional secondary swelling seen in control lymphocytes. The data suggest that N-ethylmaleimide acts on ion transport mechanisms involved in volume regulatory changes. This effect contrasts with the stimulation by N-ethylmaleimide of apparently volume sensitive K Cl fluxes in certain mamalian red cells.