Mechanical loading modulates intracellular calcium signaling in human mesenchymal stem cells.
Mechanical loading modulates intracellular calcium signaling in human mesenchymal stem cells.
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DOI：
10.5301/jabb.2008.4831
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发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
J. Campbell;D. Bader;D. Lee
中科院分区：
文献类型：
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作者：
J. Campbell;D. Bader;D. Lee
间充质干细胞（MSCs）是一种有前途的组织工程结缔组织修复策略的细胞来源。此外，越来越多的证据证实了机械环境在维持组织稳态中的作用，其中钙信号传导在机械转导途径中作为介体。自发的细胞内钙信号在4%藻酸盐水凝胶结构中包埋的MSC的子集中观察到，通过Ca 2+指示剂fluo-4结合共聚焦激光扫描显微镜测量。通过使用成对分析，显示了不同的MSC群体上调和下调钙瞬变的频率，其中施加20分钟持续时间的20%静态单轴压缩应变，在20分钟未应变期后递送。在两个未应变的20分钟期间监测对照样本中的钙瞬变。经独立性卡方检验，这些值具有统计学显著性（p<0.05）。这种双重反应指标突出了MSC群体的异质性，这可能对其在细胞疗法中的成功应用具有重要意义。
Mesenchymal stem cells (MSCs) are a promising cell source for tissue-engineered connective tissue repair strategies. Additionally, increasing evidence confirms the role of the mechanical environment in maintaining tissue homeostasis, with calcium signaling implicated as a mediator in mechanotransduction pathways. Spontaneous intracellular calcium signaling was observed in a subset of MSCs embedded within 4% alginate hydrogel constructs, measured by a Ca2+ indicator fluo-4 in conjunction with confocal laser-scanning microscopy. By the use of pair-wise analysis, it was shown that distinct populations of MSCs up-regulated and down-regulated the frequency of calcium transients with the application of a 20% static uniaxial compressive strain of 20 min duration, delivered after a 20 min unstrained period. Calcium transients in control specimens were monitored throughout two unstrained 20 min periods. These values were statistically significant (p<0.05) by chi 2 test of independence. This dual-response indicator highlights the heterogeneous nature of MSC populations, which may have important implications for their successful use in cell therapies.