Proteins S7, S10, S16 and S19 of the human 40S ribosomal subunit are most resistant to dissociation by salt
Proteins S7, S10, S16 and S19 of the human 40S ribosomal subunit are most resistant to dissociation by salt
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DOI：
10.1016/s0167-4781(00)00252-9
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发表时间：
2000-12-01
期刊：
影响因子：
--
通讯作者：
Karpova, GG
中科院分区：
文献类型：
--
作者：
Malygin, AA;Shaulo, DD;Karpova, GG
人40 S核糖体亚基的蛋白质组分通过在0.5 M KCl存在下在蔗糖和LiCl的梯度中离心而解离。通过SDS-PAGE和2D-PAGE分析分离的蛋白质。确定了蛋白质解离的顺序，取决于盐浓度（从0.8 M至1.55 M）。大多数蛋白质在单价阳离子浓度为0.8M时开始同时分裂。发现10种蛋白质更具抗性;这些蛋白质中S7、S10、S16和S19保留得最强，因此可以被认为是核心蛋白。(C)2000 Elsevier Science B. V.保留所有权利。
The protein components of human 40S ribosomal subunits were dissociated by centrifugation in gradients of sucrose and LiCl in the presence of 0.5 M KCl. The proteins that split off were analyzed by SDS-PAGE and 2D-PAGE. The order of dissociation of the proteins, depending on the salt concentration (from 0.8 M to 1.55 M); was established. The majority of the proteins started to split off simultaneously at a monovalent cation concentration of 0.8 M. Ten proteins were found to be more resistant; of these proteins S7, S10, S16, and S19 were retained most strongly and thereby may be considered to be core proteins. (C) 2000 Elsevier Science B.V. All rights reserved.