Transplantation of endothelial progenitor cells transfected with VEGF165 to restore erectile function in diabetic rats
Transplantation of endothelial progenitor cells transfected with VEGF165 to restore erectile function in diabetic rats
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DOI：
10.1038/aja.2010.116
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发表时间：
2011-03-01
影响因子：
2.9
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Gou, Xin;He, Wei-Yang;Chen, Yong
研究血管内皮生长因子165(VEGF165)转基因内皮祖细胞移植到糖尿病勃起功能障碍(ED)大鼠海绵体内的效果。通过腹腔注射链脲佐菌素建立糖尿病ED大鼠模型。链脲佐菌素处理后，将三组大鼠分别经预处理后的内皮祖细胞移植到其海绵体。在海绵体内压(ICP)监测之后，我们的结果显示，与空质粒组和对照组相比，在基础状态和电刺激期间，试验组大鼠的颅内压显著升高(P<；0.01)。组织学检查显示试验组大鼠海绵体广泛新生血管形成。荧光显微镜显示，试验组中许多移植的内皮祖细胞存活下来，分化为内皮细胞，并整合到新生血管的部位。根据本研究的结果，我们得出结论：将VEGF165转基因的EPC移植到糖尿病ED大鼠的海绵体内，可以恢复勃起功能。《亚洲男科学杂志》(2011年)第13期，第332-338期；DOI：10.1038/aja.2010.116；2010年11月29日在线出版
The present study investigated the effect of transplanting endothelial progenitor cells (EPCs) transfected with the vascular endothelial growth factor gene (VEGF165) into the corpora cavernosa of rats with diabetic erectile dysfunction (ED). A rat model of diabetic ED was constructed via intraperitoneal injection of streptozotocin. After streptozotocin treatment, pre-treated EPCs from each of three groups of rats were transplanted into their corpora cavernosa. Our results, following intracavernosal pressure (ICP) monitoring, showed that ICP increased significantly among rats in the trial group when compared to the results from rats in the blank-plasmid and control groups during basal conditions and electrical stimulation (P < 0.01 for both comparisons). Histological examination revealed extensive neovascularisation in the corpora cavernosa of rats in the trial group. Fluorescence microscopy indicated that many of the transplanted EPCs in the trial group survived, differentiated into endothelial cells and integrated into the sites of neovascularisation. Based on the results of this study, we conclude that transplantation of VEGF165-transfected EPCs into the corpora cavernosa of rats with diabetic ED restores erectile function. Asian Journal of Andrology (2011) 13, 332-338; doi:10.1038/aja.2010.116; published online 29 November 2010