Anaerobic degradation of aromatic compounds coupled to Fe(III) reduction by Ferroglobus placidus.
Anaerobic degradation of aromatic compounds coupled to Fe(III) reduction by Ferroglobus placidus.
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DOI：
10.1046/j.1462-2920.2001.00192.x
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发表时间：
2001-04
影响因子：
5.1
通讯作者：
Jason M. Tor;D. Lovley
中科院分区：
文献类型：
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作者：
Jason M. Tor;D. Lovley
芳香族化合物是超嗜热微生物栖息的一些厌氧环境中有机物的重要组成部分，但超嗜热微生物代谢芳香族化合物的潜力此前尚未被描述。在这项研究中，研究了嗜热铁球菌的芳香代谢。 F. placidus 在 85°C 的厌氧培养基中生长，以多种芳香族化合物作为唯一的电子供体，以结晶性较差的 Fe(III) 氧化物作为电子受体。生长与 Fe(III) 还原同时发生。支持生长的芳香族化合物包括苯甲酸盐、苯酚、4-羟基苯甲酸盐、苯甲醛、对羟基苯甲醛和叔肉桂酸（3-苯基-2-丙烯酸）。当硝酸盐作为电子受体时，这些芳香族化合物不支持生长，尽管硝酸盐支持以 Fe(II) 或 H2 作为电子供体的该生物体的生长。苯甲酸盐和苯酚的吸收以及 Fe(III) 还原的化学计量表明 F. placidus 将这些芳香族化合物完全氧化为二氧化碳，其中 Fe(III) 是唯一的电子受体。这是古细菌可以厌氧氧化芳香族化合物的第一个例子。这些结果还首次证明，超嗜热微生物可以厌氧氧化芳香族化合物，并表明超嗜热微生物可能在高温环境中代谢芳香族化合物，例如深部热地下以及海洋和陆地热液区，其中 Fe(III) 可作为电子受体。
Aromatic compounds are an important component of the organic matter in some of the anaerobic environments that hyperthermophilic microorganisms inhabit, but the potential for hyperthermophilic microorganisms to metabolize aromatic compounds has not been described previously. In this study, aromatic metabolism was investigated in the hyperthermophile Ferroglobus placidus. F. placidus grew at 85 degrees C in anaerobic medium with a variety of aromatic compounds as the sole electron donor and poorly crystalline Fe(III) oxide as the electron acceptor. Growth coincided with Fe(III) reduction. Aromatic compounds supporting growth included benzoate, phenol, 4-hydroxybenzoate, benzaldehyde, p-hydroxybenzaldehyde and t-cinnamic acid (3-phenyl-2-propenoic acid). These aromatic compounds did not support growth when nitrate was provided as the electron acceptor, even though nitrate supports the growth of this organism with Fe(II) or H2 as the electron donor. The stoichiometry of benzoate and phenol uptake and Fe(III) reduction indicated that F. placidus completely oxidized these aromatic compounds to carbon dioxide, with Fe(III) serving as the sole electron acceptor. This is the first example of an Archaea that can anaerobically oxidize an aromatic compound. These results also demonstrate for the first time that hyperthermophilic microorganisms can anaerobically oxidize aromatic compounds and suggest that hyperthermophiles may metabolize aromatic compounds in hot environments such as the deep hot subsurface and in marine and terrestrial hydrothermal zones in which Fe(III) is available as an electron acceptor.