Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte.
Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte.
复制标题
DOI：
10.1083/jcb.126.6.1475
复制
发表时间：
1994-09
期刊：
影响因子：
--
通讯作者：
Hays TS
中科院分区：
文献类型：
--
作者：
Li M;McGrail M;Serr M;Hays TS
微管相关马达、动力蛋白和驱动蛋白的单向运动为细胞器和分子的定位提供了重要机制。一个有趣的可能性是，这一机制可能是影响多细胞胚胎发育的形态发生素的定向运输和不对称定位的基础。在这份报告中，我们的特点果蝇基因，Dhc64C，编码细胞质动力蛋白重链多肽。果蝇细胞质动力蛋白重链多肽的一级结构已通过重叠cDNA克隆的分离和序列分析确定。果蝇细胞质动力蛋白在序列和结构上与其他生物体的细胞质动力蛋白亚型高度相似。Dhc64C动力蛋白转录本在发育过程中差异表达，在成年雌性的卵巢中检测到最高水平。在卵巢发育的卵室内，动力蛋白基因主要在滋养细胞复合体中转录。相反，编码动力蛋白的马达蛋白在将发育为卵母细胞的单个细胞中显示出惊人的积累。卵母细胞中动力蛋白积累的时间和空间模式与卵母细胞分化和轴特化所必需的几种母体效应基因产物非常相似。这种分布和它的破坏特定的母体效应突变借给支持最近的模型表明，微管电机参与运输这些形态从护士细胞的细胞质的卵母细胞。
The unidirectional movements of the microtubule-associated motors, dyneins, and kinesins, provide an important mechanism for the positioning of cellular organelles and molecules. An intriguing possibility is that this mechanism may underlie the directed transport and asymmetric positioning of morphogens that influence the development of multicellular embryos. In this report, we characterize the Drosophila gene, Dhc64C, that encodes a cytoplasmic dynein heavy chain polypeptide. The primary structure of the Drosophila cytoplasmic dynein heavy chain polypeptide has been determined by the isolation and sequence analysis of overlapping cDNA clones. Drosophila cytoplasmic dynein is highly similar in sequence and structure to cytoplasmic dynein isoforms reported for other organisms. The Dhc64C dynein transcript is differentially expressed during development with the highest levels being detected in the ovaries of adult females. Within the developing egg chambers of the ovary, the dynein gene is predominantly transcribed in the nurse cell complex. In contrast, the encoded dynein motor protein displays a striking accumulation in the single cell that will develop as the oocyte. The temporal and spatial pattern of dynein accumulation in the oocyte is remarkably similar to that of several maternal effect gene products that are essential for oocyte differentiation and axis specification. This distribution and its disruption by specific maternal effect mutations lends support to recent models suggesting that microtubule motors participate in the transport of these morphogens from the nurse cell cytoplasm to the oocyte.
登录
查看更多内容
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
64.8
作者：
GIBBONS, IR;GIBBONS, BH;ASAI, DJ
通讯作者：
ASAI, DJ
影响因子：
64.5
作者：
CARR, CM;KIM, PS
通讯作者：
KIM, PS
DOI：
10.1083/jcb.122.1.113
发表时间：
1993-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baker J;Theurkauf WE;Schubiger G
通讯作者：
Schubiger G
影响因子：
5.6
作者：
GARNIER, J;OSGUTHORPE, DJ;ROBSON, B
通讯作者：
ROBSON, B