Induction of a robust humoral response using HIV-1 VLPMPER-V3as a novel candidate vaccine in BALB/c mice.
Induction of a robust humoral response using HIV-1 VLPMPER-V3as a novel candidate vaccine in BALB/c mice.
复制标题
使用 HIV-1 VLPMPER-V3 作为 BALB/c 小鼠的新型候选疫苗诱导强烈的体液反应。
DOI：
10.2174/1570162x17666190306124218
复制
发表时间：
2019
影响因子：
1
通讯作者：
M. S. Larijani
中科院分区：
文献类型：
--
作者：
F. Tohidi;S. M. Sadat;A. Bolhassani;R. Yaghoubi;M. S. Larijani
背景
几种方法尚未成功地抑制感染个体中的HIV（人类免疫缺陷病毒）感染或预防它。为了扩大强的HIV特异性体液和细胞应答，作为潜在疫苗的病毒样颗粒（VLP）显示出中和抗体分泌、T细胞计数以及细胞因子分泌的显著增加。
目的
本研究的目的是在BALB/c小鼠中对携带高拷贝MPER-V3的VLP进行免疫学评价。
方法
用HIV-1 VLPMPER-V3的同源和异源初免-加强方案免疫雌性BALB/c小鼠。通过体液免疫应答（总IgG和IgG同种型）和细胞免疫应答（IFN-γIL-5分泌、体外CTL试验和T细胞增殖）来评价它们的免疫应答，并在免疫小鼠中进行比较。
结果
数据显示在接受不同VLP方案的小鼠组中强烈诱导体液应答。细胞因子谱分析表明，IL-5分泌最高的是VLP+M50组，证实了该组中Th 2免疫的优势。
结论
这项研究表明，VLP MPER-V3作为一种潜在的候选疫苗，具有作为一种有效的预防性疫苗的效力，这一发现保证了进一步的研究，以实现一个有前途的HIV-1疫苗候选。
BACKGROUND
Several approaches have not been successful to suppress HIV (Human immunodeficiency virus) infection among infected individuals or to prevent it yet. In order to expand strong HIV specific humoral and cellular responses, Virus-like particles (VLPs) as potential vaccines show significant increase in neutralizing antibodies secretion, T-cell count and also secretion of cytokines.
OBJECTIVE
This study aimed at immunological evaluation of VLPs harboring high copy of MPER-V3 in BALB/c mice.
METHOD
Female BALB/c mice were immunized with homologous and heterologous prime-boosting regimens of HIV-1 VLPMPER-V3. Their immune responses were evaluated for humoral responses (Total IgG and IgG isotyping) and cellular responses (IFN-γIL-5 secretion, in vitro CTL assay and T cell proliferation) and compared in immunized mice.
RESULTS
The data was shown robust induction of humoral response in mice groups which received different regimens of VLP. Furthermore, analysis of cytokine profile indicated that the highest IL-5 secretion was related to VLP+M50 group and confirmed the dominancy of Th2 immunity in this group.
CONCLUSION
This study showed that VLP MPER-V3 as a potential vaccine candidate has the potency as an effective prophylactic vaccine and this finding guarantees further investigations to achieve a promising HIV-1 vaccine candidate.
含有修饰的人类免疫缺陷病毒包膜蛋白的病毒样颗粒的免疫原性。
影响因子：
5.5
作者：
Quan,Fu-Shi;Sailaja,Gangadhara;Skountzou,Ioanna;Huang,Chunzi;Vzorov,Andrei;Compans,RichardW;Kang,Sang-Moo
通讯作者：
Kang,Sang-Moo
影响因子：
20.3
作者：
Cheong, Cheolho;Choi, Jae-Hoon;Park, Chae Gyu
通讯作者：
Park, Chae Gyu
影响因子：
1.5
作者：
Kuerten, Stefanie;Nowacki, Tobias M.;Tary-Lehmann, Magdalena
通讯作者：
Tary-Lehmann, Magdalena