Electron Paramagnetic Resonance Investigation on Modulatory Effect of 17&bgr;-Estradiol on Membrane Fluidity of Erythrocytes in Postmenopausal Women
Electron Paramagnetic Resonance Investigation on Modulatory Effect of 17&bgr;-Estradiol on Membrane Fluidity of Erythrocytes in Postmenopausal Women
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电子顺磁共振研究17的调制作用
DOI：
10.1161/hq0801.093507
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Y. Masuyama
中科院分区：
文献类型：
--
作者：
K. Tsuda;Y. Kinoshita;K. Kimura;I. Nishio;Y. Masuyama
摘要：许多研究表明，雌激素可以发挥心脏保护作用，降低高血压和冠状动脉事件的风险。另一方面，已经提出细胞膜异常在高血压的病理生理学中起作用，尽管尚不清楚雌激素是否会影响原发性高血压的膜功能。本研究旨在探讨17-雌二醇（E2）对绝经后正常和高血压妇女红细胞膜流动性的影响。我们用电子顺磁共振和自旋标记法测定了红细胞膜流动性。在体外研究中，E2显着降低了5-硝基氧硬脂酸酯的顺序参数和16-硝基氧硬脂酸酯的峰高比从血压正常的绝经后妇女的红细胞膜的电子顺磁共振光谱。提示E_2可增加红细胞膜流动性。NO供体S-亚硝基-N-乙酰青霉胺和cGMP类似物8-溴-cGMP可显著增强E2的作用。与此相反，由E2引起的膜流动性的变化减弱的NO合成酶抑制剂，NG-硝基-L-精氨酸甲酯，和不对称的二甲基-L-精氨酸的存在下。绝经后高血压妇女红细胞膜流动性明显低于绝经后正常血压妇女。E2对膜流动性的影响在高血压绝经后妇女的红细胞中比在血压正常绝经后妇女的红细胞中更明显。本研究的结果表明，E2显着增加膜流动性和改善红细胞膜的微粘度，部分介导的NO和cGMP依赖的途径。此外，E2在高血压中的更大作用可能与E2在调节红细胞流变学行为方面具有有益作用的假设一致，并且可能在高血压的微循环改善中起关键作用。
Abstract—Many studies have shown that estrogen may exert cardioprotective effects and reduce the risk of hypertension and coronary events. On the other hand, it has been proposed that cell membrane abnormalities play a role in the pathophysiology of hypertension, although it is not clear whether estrogen would influence membrane function in essential hypertension. The present study was performed to investigate the effects of 17&bgr;-estradiol (E2) on membrane fluidity of erythrocytes in normotensive and hypertensive postmenopausal women. We determined the membrane fluidity of erythrocytes by means of an electron paramagnetic resonance and spin-labeling method. In an in vitro study, E2 significantly decreased the order parameter for 5-nitroxide stearate and the peak height ratio for 16-nitroxide stearate obtained from electron paramagnetic resonance spectra of erythrocyte membranes in normotensive postmenopausal women. The finding indicates that E2 might increase the membrane fluidity of erythrocytes. The effect of E2 was significantly potentiated by the NO donor, S-nitroso-N-acetylpenicillamine, and a cGMP analogue, 8-bromo-cGMP. In contrast, the change in the membrane fluidity evoked by E2 was attenuated in the presence of the NO synthase inhibitor, NG-nitro-l-arginine methyl ester, and asymmetric dimethyl-l-arginine. In hypertensive postmenopausal women, the membrane fluidity of erythrocytes was significantly lower than that in normotensive postmenopausal women. The effect of E2 on membrane fluidity was significantly more pronounced in the erythrocytes of hypertensive postmenopausal women than in the erythrocytes of normotensive postmenopausal women. The results of the present study showed that E2 significantly increased the membrane fluidity and improved the microviscosity of erythrocyte membranes, partially mediated by an NO- and cGMP-dependent pathway. Furthermore, the greater action of E2 in hypertension might be consistent with the hypothesis that E2 could have a beneficial effect in regulating rheological behavior of erythrocytes and could have a crucial role in the improvement of the microcirculation in hypertension.
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影响因子：
8.3
作者：
E. Seely;B. Walsh;M. Gerhard;Gordon H. Williams
通讯作者：
E. Seely;B. Walsh;M. Gerhard;Gordon H. Williams
DOI：
10.1097/00006254-199202000-00027
发表时间：
1991-09
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Stampfer;G. Colditz;W. Willett;J. Manson;B. Rosner;F. Speizer;C. Hennekens
通讯作者：
M. Stampfer;G. Colditz;W. Willett;J. Manson;B. Rosner;F. Speizer;C. Hennekens
在高胰岛素血症高血压受试者中，钠-锂逆向转运对钠的亲和力较低。
DOI：
10.1161/01.hyp.25.5.986
发表时间：
1995
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Zerbini,G;Ceolotto,G;Gaboury,C;Mos,L;Pessina,AC;Canessa,M;Semplicini,A
通讯作者：
Semplicini,A
DOI：
10.1152/ajpheart.1997.272.6.h2765
发表时间：
1997-06-01
影响因子：
4.8
作者：
Darkow, DJ;Lu, L;White, RE
通讯作者：
White, RE