Expression and Functions of SARS Coronavirus Replicative Proteins
Expression and Functions of SARS Coronavirus Replicative Proteins
复制标题
DOI：
10.1007/978-3-642-03683-5_6
复制
发表时间：
2009-07-22
期刊：
影响因子：
--
通讯作者：
Ziebuhr J
中科院分区：
文献类型：
--
作者：
Ulferts R;Imbert I;Canard B;Ziebuhr J
2002/2003年SARS流行的病原体是一种以前未知的冠状病毒，这一发现刺激了对SARS冠状病毒（SARS-CoV）及其相关病毒的分子生物学研究。这项研究为冠状病毒复制酶-转录酶复合物的功能和活性提供了重要的新见解，该复合物是一种多蛋白复合物，可指导连续和不连续RNA合成的协调过程，以复制和转录大型冠状病毒基因组，这是一种约30 kb的单链正义RNA。在本章中，我们回顾了我们目前对关键复制酶的表达和功能的理解，如RNA聚合酶，解旋酶，核糖核酸酶，核糖-2 ′-O-甲基转移酶和其他复制酶基因编码的蛋白质参与基因组表达，病毒-宿主相互作用和其他过程。总的来说，这些最近的研究揭示了酶机制的迷人细节，在RNA病毒世界中，就病毒复制和宿主相互作用所涉及的病毒编码活动的数量和性质而言，酶机制是无与伦比的。
The discovery of a previously unknown coronavirus as the causative agent of the SARS epidemic in 2002/2003 stimulated a large number of studies into the molecular biology of SARS coronavirus (SARS-CoV) and related viruses. This research has provided significant new insight into the functions and activities of the coronavirus replicase–transcriptase complex, a multiprotein complex that directs coordinated processes of both continuous and discontinuous RNA synthesis to replicate and transcribe the large coronavirus genome, a single-stranded, positive-sense RNA of ~30 kb. In this chapter, we review our current understanding of the expression and functions of key replicative enzymes, such as RNA polymerases, helicase, ribonucleases, ribose-2′-O-methyltransferase and other replicase gene-encoded proteins involved in genome expression, virus–host interactions and other processes. Collectively, these recent studies reveal fascinating details of an enzymatic machinery that, in the RNA virus world, is unparalleled in terms of the number and nature of virally encoded activities involved in virus replication and host interactions.