Progesterone receptor as an indicator of sperm function
Progesterone receptor as an indicator of sperm function
复制标题
DOI：
10.1095/biolreprod67.4.1327
复制
发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Puri, CP
中科院分区：
文献类型：
--
作者：
Gadkar, S;Shah, CA;Puri, CP
本文测定了正常和异常精子图男性精子孕酮受体（PR）的表达。还进行了研究，以评估PR作为精子功能标志物的潜力。采用抗PR单克隆抗体免疫细胞化学法和流式细胞术分析了正常精子症（n = 8）、少精子症（n = 7）、弱精子症（n = 8）、少弱精子症（n = 7）和畸形精子症（n = 11）男性精子上的孕激素受体表达。两种方法均显示少精子症、弱精子症、少弱精子症和畸形精子症患者PR阳性精子数显著低于正常精子症患者（P < 0.05），提示精子PR表达下调可能是男性不育的原因之一。将来自正常精子症（n = 12）、少精子症（n = 12）、弱精子症（n = 12）、少弱精子症（n = 9）和畸形精子症（n = 10）男性的精子在低渗肿胀（HOS）试验中暴露于低渗条件，然后使用P-FITCBSA复合物分析PR表达。在所有类型的不育男性中，具有生理活性质膜（HOS+）的精子缺乏PR表达（HOS+PR-）的百分比显著较高（P < 0.05），从而表明与HOS测试相比，PR表达是精子功能的更好指标。此外，精子中PR的表达与其进行体外顶体反应的能力呈强正相关（P < 0.05）。这在所有研究组中都观察到（即，正常精子症r = 0.8545，少精子症r = 0.8711，弱精子症r = 0.7645，少弱精子症r = 0.9003，畸形精子症r = 0.8676。这表明PR在导致精子顶体反应的事件中具有潜在作用。
Expression of progesterone receptor (PR) localization on spermatozoa was determined in men with normal and abnormal spermiograms. Studies were also carried out to evaluate the potential of PR as a marker of sperm function. Progesterone receptor expression on spermatozoa from men with normozoospermia (n = 8), oligozoospermia (n = 7), asthenozoospermia (n = 8), oligoasthenozoospermia (n = 7), and teratozoospermia (n = 11) was analyzed using an immunocytochemical method with monoclonal antibodies against PR, and flow cytometry using a cell-impermeable fluorescein-tagged progesterone coupled to BSA complex (P-FITC-OSA). Both methods revealed significantly fewer (P < 0.05) PR-positive spermatozoa in men with oligozoospermia, asthenozoospermia, oligoasthenozoospermia, and teratozoospermia compared with men with normozoospermia, thereby suggesting that down-regulation of PR expression in spermatozoa may be one of the causes of male infertility. Spermatozoa from men with normozoospermia (n = 12), oligozoospermia (n = 12), asthenozoospermia (n = 12), oligoasthenozoospermia (n = 9), and teratozoospermia (n = 10) were exposed to low osmotic conditions in the hypoosmotic swelling (HOS) test and then analyzed for PR expression using P-FITCBSA complex. A significantly higher percentage (P < 0.05) of spermatozoa with physiologically active plasma membrane (HOS+) lacked PR expression (HOS+PR-) in all categories of men with infertility, thereby suggesting that compared to the HOS test, PR expression is a better indicator of sperm function. Furthermore, PR expression in spermatozoa showed a strong (P < 0.05) positive correlation with their ability to undergo an in vitro acrosome reaction. This was observed in all study groups (i.e., normozoospermia, r = 0.8545; oligozoospermia, r = 0.8711; asthenozoospermia, r = 0.7645; oligoasthenozoospermia, r = 0.9003; and teratozoospermia, r = 0.8676). This suggests a potential role for PR in the events leading to the acrosome reaction in sperm.